PMID: 8972727Nov 8, 1996Paper

Stimulation of glucose transport in Clone 9 cells by insulin and thyroid hormone: role of GLUT-1 activation

Biochimica Et Biophysica Acta
M ShettyJ N Loeb

Abstract

Thyroid hormone (T3) and insulin are both shown to stimulate glucose transport in Clone 9 cells, a rat liver cell line in which the utilization of glucose is limited by transport rate and in which only the GLUT-1 transporter isoform is expressed. Pre-treatment of these cells with T3 moreover substantially enhances the stimulatory effect of insulin such that at maximally effective hormone concentrations the effects of T3 and insulin on glucose transport are more than additive and indeed nearly multiplicative, suggesting that the mechanisms mediating the enhancement of glucose transport differ between the two hormones. Cell surface biotinylation followed by Western-blot analysis of plasma membrane fractions showed that the stimulatory effects of T3 and insulin on glucose transport, whether acting singly or in combination, exceed the attendant increases in the abundance of GLUT-1 in the plasma membrane. It is suggested that activation of GLUT-1 molecules pre-existing in the plasma membrane plays a major role in mediating the stimulatory effects of T3 and insulin on glucose transport in this cell line.

References

Jan 1, 1992·Annual Review of Physiology·J E Pessin, G I Bell
Sep 24, 1991·Biochimica Et Biophysica Acta·A K KuruvillaJ N Loeb
Jan 1, 1990·Endocrinology·P F Pilch
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Jan 1, 1993·Thyroid : Official Journal of the American Thyroid Association·S P Weinstein, R S Haber

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Citations

Jan 5, 1999·European Journal of Pharmacology·J A García-SáinzC González-Espinosa
Dec 24, 1997·Molecular and Cellular Endocrinology·J YbarraF Ismail-Beigi
Nov 3, 2009·Biochemical Pharmacology·Araceli Arellano-PlancarteJ Alberto Olivares-Reyes
Jun 21, 2016·Biochemical and Biophysical Research Communications·Danielle M DefriesPeter Zahradka
Oct 21, 2016·Cell and Tissue Research·Yu DingCheng Zhang
Jan 29, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Luc Leybaert
Apr 5, 2007·American Journal of Physiology. Endocrinology and Metabolism·Bernard R LandauFaramarz Ismail-Beigi
Apr 11, 2003·American Journal of Physiology. Cell Physiology·Darrell Rubin, Faramarz Ismail-Beigi
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Klaus Lange
Sep 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Mary M NellisDean J Danner
Mar 13, 2019·European Journal of Nuclear Medicine and Molecular Imaging·Ellen BoswijkJan Bucerius
Mar 13, 2002·Archives of Biochemistry and Biophysics·Daw-Yang Hwang, Faramarz Ismail-Beigi
Jul 13, 1999·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J Z ZhangF Ismail-Beigi

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