Stimulation of group II phospholipase A2 mRNA expression and release in an immortalized astrocyte cell line (DITNC) by LPS, TNF alpha, and IL-1 beta. Interactive effects

Molecular and Chemical Neuropathology
W TongG Y Sun

Abstract

Astrocytes are immunoactive cells in brain and have been implicated in the defense mechanism in response to external injury. Previous studies using cultured glial cells indicated the ability of astrocytes to respond to bacteria endotoxin and cytokines, resulting in the release of phospholipase A2. In this study, we examined the interactive effects of lipopolysaccharides (LPS), interleukin 1 beta (IL-1 beta) and tumor necrosis factor (TNF alpha) to stimulate phospholipase A2 (PLA2) in an immortalized astrocyte cell line (DITNC) with many properties of type I astrocytes. Northern blot analysis using oligonucleotide probes derived from the cDNA encoding the rat spleen group II PLA2 indicated the ability of DITNC cells to respond to all three factors in the induction of gene expression and the release of PLA2. After an initial lag time of 2 h, PLA2 release was proportional to time, reaching a plateau by 12 h. This event occurred at a time period preceding any signs of cell death. Cycloheximide at 1.25 microM completely inhibited cytokine-induced PLA2 release. When suboptimal amounts of TNF alpha were added to the DITNC culture together with IL-1 beta or LPS, a synergistic increase in the induction of PLA2 release could be observed....Continue Reading

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Citations

Mar 20, 2010·Molecular Neurobiology·Gianfrancesco GoracciVincenza Nardicchi
Nov 19, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J XuG Y Sun
Jun 15, 1999·Molecular Medicine Today·L Touqui, L Arbibe
Mar 25, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·W LiG Y Sun
Mar 12, 2015·Biochemical and Biophysical Research Communications·Wonhwa LeeJong-Sup Bae
Jun 29, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Moon-Chang BaekJong-Sup Bae
Dec 6, 2003·Journal of Lipid Research·Grace Y SunAgnes Simonyi
May 14, 2005·Biotechnology and Bioengineering·Hisashi SatohTakuya Suzuki
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A BretscherD Reczek
Feb 15, 2001·Journal of Biomedical Science·A Y Sun, G Y Sun
Jun 30, 2021·Neurochemistry International·Ivan A LindhoutAndis Klegeris

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