PMID: 6113847Apr 14, 1981Paper

Stimulation of guanylate cyclase by EDTA and other chelating agents

Biochimica Et Biophysica Acta
W H FreyS E Nicol

Abstract

The partially purified soluble guanylate cyclase (GTP pyrophosphatelyase(cyclizing), EC 4.6.1.2) from human caudate nucleus is stimulated from 2 to 4-fold by metal chelating agents. EDTA (K 1/2 - 4.8 microM) is more potent than CDTA (K 1/2 = 13.2 microM) or EGTA (K 1/2 = 21.8 microM) at stimulating activity. Stimulation by chelating agents is apparently not due to removal of inhibitory divalent cations which contaminate the enzyme or reaction mixture. EDTA increases guanylate cyclase activity in part by increasing the affinity of the enzyme for the substrate (MgGTP) 10-fold. Dopamine inhibits partially purified guanylate cyclase in the presence or absence of EDTA. Dopamine increases the Ka of guanylate cyclase for the activator, free Mn2+, more than 50-fold, from 3 to 150 microM.

References

May 1, 1976·Life Sciences·D R OlsonB M Breckenridge
Jan 1, 1976·Analytical Biochemistry·H L CaillaM A Delaage
Mar 1, 1975·Metabolism: Clinical and Experimental·H Kimura, F Murad
Nov 1, 1973·Archives of Biochemistry and Biophysics·W J ThompsonS A Little
Jul 16, 1973·European Journal of Biochemistry·D von Hungen, S Roberts
Aug 1, 1974·Journal of Neurochemistry·P KleihuesK A Hossmann
Dec 1, 1980·Journal of Neurochemistry·W H FreyS E Nicol

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.