Jan 1, 1978

Stimulation of heme accumulation and erythroid colony formation in cultures of chick bone marrow cells by chicken plasma

The Journal of Cell Biology
J Coll, V M Ingram


A fibrin clot culture system with high plating efficiency is described for the growth of erythroid cells from chick bone marrow. Erythroid colonies grown in the absence of adult chicken plasma (spontaneous colonies) were either benzidine-negative or weakly benzidine-positive. Colonies grown in the presence of chicken plasma were 90% strongly benzidine-positive and 40% more abundant than spontaneous colonies. Plasma from anemic chickens was more effective than control plasma in inducing heme accumulation (heme-stimulating activity) and in increasing the number of erythroid colonies (colony-stimulating activity). Spontaneous colonies from 48-h cultures were transformed into benzidine-positive colonies by exposing them for 6-10 h to chicken plasma.

  • References4
  • Citations5


Mentioned in this Paper

Fresh Frozen Plasma
Cell Differentiation Process
Staining and Labeling

About this Paper

Related Feeds


Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.