Stimulation of human endothelium with IL-3 induces selective basophil accumulation in vitro

The Journal of Immunology : Official Journal of the American Association of Immunologists
Lina H K LimB S Bochner

Abstract

Basophils have been shown to accumulate in allergic airways and other extravascular sites. Mechanisms responsible for the selective recruitment of basophils from the blood into tissue sites remain poorly characterized. In this study, we characterized human basophil rolling and adhesion on HUVECs under physiological shear flow conditions. Interestingly, treatment of endothelial cells with the basophil-specific cytokine IL-3 (0.01-10 ng/ml) promoted basophil and eosinophil, but not neutrophil, rolling and exclusively promoted basophil adhesion. Preincubation of HUVECs with an IL-3R-blocking Ab (CD123) before the addition of IL-3 inhibited basophil rolling and adhesion, implicating IL-3R activation on endothelial cells. Incubation of basophils with neuraminidase completely abolished both rolling and adhesion, indicating the involvement of sialylated structures in the process. Abs to the beta(1) integrins, CD49d and CD49e, as well as to P-selectin and P-selectin glycoprotein ligand 1, inhibited basophil rolling and adhesion. Furthermore, blocking chemokine receptors expressed by basophils, such as CCR2, CCR3, and CCR7, demonstrated that CCR7 was involved in the observed recruitment of basophils. These data provide novel insights in...Continue Reading

References

Mar 1, 1992·The Journal of Clinical Investigation·R AlamJ A Grant
Sep 1, 1992·The Journal of Experimental Medicine·R AlamJ A Grant
Oct 1, 1994·American Journal of Respiratory and Critical Care Medicine·N MaruyamaT Takishima
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·S ChenT A Springer
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·L H LimM Perretti
May 4, 1999·International Archives of Allergy and Immunology·M H GraysonB S Bochner
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H TachimotoB S Bochner
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K L DavenpeckB S Bochner
Nov 18, 2000·The Journal of Allergy and Clinical Immunology·M L TaylorB S Bochner
May 9, 2001·The Journal of Experimental Medicine·E S BaekkevoldG Haraldsen
May 10, 2001·The Journal of Allergy and Clinical Immunology·S S SainiD W Macglashan
Dec 13, 2002·American Journal of Physiology. Cell Physiology·Monica M BurdickKonstantinos Konstantopoulos
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wolfgang WeningerUlrich H von Andrian
Oct 8, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Motoyasu IikuraKoichi Hirai

❮ Previous
Next ❯

Citations

Jan 21, 2009·Allergology International : Official Journal of the Japanese Society of Allergology·Masao YamaguchiMotoyasu Iikura
Oct 17, 2007·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Yumiko Yasui Nishii, Masaaki Akagi
Nov 29, 2007·Current Opinion in Hematology·Booki Min, William E Paul
Dec 26, 2018·Frontiers in Physiology·Hajime KarasuyamaSoichiro Yoshikawa
Feb 21, 2008·Nature Immunology·Booki Min, William E Paul
Jan 13, 2017·BioMed Research International·Markus SteinerMartin Himly
May 10, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Saidou BalamMatthias Mack
Oct 31, 2020·Parasite Immunology·Soichiro YoshikawaHajime Karasuyama
Jan 18, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lina HanMarina Konopleva
Nov 7, 2020·Frontiers in Immunology·Hajime KarasuyamaSoichiro Yoshikawa
Aug 20, 2021·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Zijun ZhaoEric R Tkaczyk
Apr 8, 2009·The Journal of Allergy and Clinical Immunology·Niya WanichWayne G Shreffler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
P SriramaraoD H Broide
The Journal of Immunology : Official Journal of the American Association of Immunologists
B S BochnerD W MacGlashan
The Journal of Immunology : Official Journal of the American Association of Immunologists
Bouchaib LamkhiouedPaolo M Renzi
The Journal of Immunology : Official Journal of the American Association of Immunologists
F A SymonA J Wardlaw
© 2022 Meta ULC. All rights reserved