Stimulation of interleukin-1beta-independent interleukin-6 production in human dental pulp cells by lipopolysaccharide

Biochemical and Molecular Medicine
S HosoyaY Abiko

Abstract

Dental pulpal infection is most commonly caused by extensive dental caries. A principal driving force behind pulpal disease response appears to lie in the immune system's response to bacteria. However, the production of interleukin (IL)-1beta and IL-6 in human dental pulp (HDP) cells in response to lipopolysaccharide (LPS) has not been well characterized. We examined IL-1beta and IL-6 production in HDP cells by challenging with LPS from Porphyromonas endodontalis, which is a Gram-negative bacteria found in root canals. Our results presented here showed that when HDP cells were stimulated by LPS, the production of IL-6 always preceded that of IL-1beta. Since the IL-6 production was observed even in the presence of the IL-1beta receptor antagonist, we concluded IL-6 production was independent of the IL-1beta molecule in LPS-stimulated HDP cells. This idea was further supported by the results obtained from RT-PCR experiments, in which IL-6 mRNA, but not IL-1beta mRNA, was present in the RNA preparation isolated from the early stage of cells.

Citations

Jun 11, 1999·Journal of Internal Medicine·S SöderbergB Ahrén
Dec 12, 2001·Oral Microbiology and Immunology·G RosenM N Sela
Jun 29, 2010·Journal of Dentistry·Paul R CooperAnthony J Smith
Nov 25, 2003·American Journal of Reproductive Immunology : AJRI·N D QuintonA I F Blakemore
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Feb 24, 2001·Journal of Endodontics·R NissanR Stevens
May 6, 2008·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Masaru YamaguchiKazutaka Kasai
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