Abstract
Although growth cones respond to various modulators of neurite outgrowth, such as neurotrophins, neurotransmitters, and cell adhesion molecules, the signal-transducing mechanisms for these modulators in growth cones are unclear. Since recent studies have suggested that the signals of these modulators are mediated by Ca2+ influx through L-type voltage-sensitive Ca2+ channels (VSCCs) in the growth cone, we examined L-type VSCC-dependent signaling pathways, using isolated growth cones (IGCs) from developing rat forebrains. Binding assays revealed that L-type VSCC is enriched in growth cone membrane and gradually decreased in amount developmentally, while N-type VSCC has the opposite tendency. In intact IGCs, Bay K 8644 (BK, an L-type agonist) induced much more rapid elevation of [Ca2+]i than that in adult synaptosomes. Ca2+-dependent phosphorylation of GAP-43 and MARCKS protein by protein kinase C (PKC) was enhanced in the IGC by BK, resulting in the release of these proteins from the membrane, which is consistent with our recent report. In addition, the Ca2+-dependent degradation of brain spectrin (fodrin) by calpain was also enhanced by BK or GABA, consequently inducing the release of alpha-actinin from the membrane skeleton of ...Continue Reading
Citations
Sep 15, 2007·The Journal of Clinical Investigation·Jason S GroshongChristopher M Gomez
Dec 18, 2013·Cell Communication and Signaling : CCS·Lifu ShengVladimir Sytnyk
Sep 24, 2008·The Journal of Cell Biology·Vsevolod BodrikovMelitta Schachner
Apr 20, 2004·The European Journal of Neuroscience·Gerald J ObermairBernhard E Flucher
Apr 9, 2010·The European Journal of Neuroscience·Chessa S ScullinL Donald Partridge
Oct 24, 2002·Neurological Research·Agahan UnluBermans J Iskandar
Feb 13, 2001·Experimental Neurology·M FountoulakisG Lubec
Nov 22, 2000·Molecular and Cellular Neurosciences·P DohertyE J Williams
Jun 22, 2000·Journal of Neuroscience Research·M IgarashiY Komiya
Aug 11, 2004·Hippocampus·Takashi TokunagaHiromu Yawo
Aug 24, 2007·The European Journal of Neuroscience·Kristy Welshhans, Vincent Rehder
Nov 26, 2009·Stress : the International Journal on the Biology of Stress·Alexandra KavushanskyEhud Klein
Dec 17, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Makoto HiguchiTakaomi C Saido
Dec 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vitaliy StarostaAlexander Kurosky
Oct 25, 2001·Brain Research. Developmental Brain Research·F R Zolessi, C Arruti
Nov 9, 2000·Neuroscience·Q ChenR D Brinton
Dec 28, 2000·Progress in Neuro-psychopharmacology & Biological Psychiatry·H FukuraM Igarashi
May 30, 2001·Neuroscience·T Tojima, E Ito
Apr 7, 2000·Brain Research. Brain Research Reviews·M F Mehler