Stimulation of lactic acid production in chick embryo fibroblasts by serum and high pH in the absence of external glucose

Journal of Cellular Physiology
D W Fodge, H Rubin

Abstract

Lactic acid production by chick embryo fibroblasts occurs in the absence of exogenous glucose. Fifteen to 50-fold less lactic acid is formed in the absence of glucose than in its presence. Nevertheless, serum and pH stimulation enhances this residual lactic acid production to the same relative extent as when glucose is present. The amount of lactic acid formed cannot be accounted for by the catabolism of residual glucose in the medium since its concentration is less than one-tenth that of the lactic acid eventually produced. Moreover, the residual glucose concentration remains constant or increases during the course of the experiment. To a large extent lactic acid accumulation in the absence of external glucose is dependent on the presence of amino acids in the medium, but amino acid transport is not affected by the stimulatory agents used in this study. The results suggest that treatments which stimulate cell multiplication also activate those enzymatic pathways which convert amino acids to pyruvic and thence to lactic acid.

References

Aug 1, 1975·Journal of Cellular Physiology·H Rubin, T Koide
Dec 1, 1971·The Journal of Cell Biology·H Rubin
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S Venuta, H Rubin
Dec 12, 1973·Nature: New Biology·D W Fodge, H Rubin

Citations

Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H R ZielkeM Cornblath
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H RübsamenE Eigenbrodt
Mar 1, 1982·International Journal of Radiation Oncology, Biology, Physics·C F ChaoR J Johnson
Mar 4, 2006·Biotechnology and Bioengineering·A S CoroadinhaM J T Carrondo
Dec 1, 1981·Experimental Cell Research·P T Van der SaagS W De Laat
Jan 1, 1983·Journal of Cellular Physiology·E J Kasambalides, K W Lanks
Sep 1, 1981·Bioscience Reports·M J Morgan, P Faik
Apr 1, 1978·Journal of Cellular Physiology·H R ZielkeM Cornblath
Aug 1, 1989·Experimental Cell Research·C WolfromM Gautier
Jan 1, 1978·Journal of Cellular Physiology·A H Rubin, B Chu

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