Stimulation of liver regeneration after hepatectomy in mice by injection of bone marrow mesenchymal stem cells via the portal vein

Transplantation Proceedings
M KaiboriA H Kwon

Abstract

To investigate whether mouse bone marrow mesenchymal stem cells (BMC) stimulate liver regeneration after partial hepatectomy. Isolated BMCs were purified by density gradient centrifugation. We performed a 70% hepatectomy in male BALB/c mice followed by injection of BMCs into the portal vein (PV-BMC group), or the tail vein (IV-BMC group), or of saline into the portal vein (control group). The wet weight of the liver remnant increased significantly in the PV-BMC group at 3 and 5 days after hepatectomy compared with the IV-BMC and control groups. The Ki-67 labeling index revealed that the increase to result from stimulation of DNA synthesis. The constitutive interleukin-6 and hepatocyte growth factor mRNAs in the remnant liver tended to increase in the PV-BMC group at 3 days after hepatectomy. These results demonstrated that BMC injection into the portal vein enhanced liver growth after partial hepatectomy in mice.

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Citations

Jan 24, 2014·Digestive Diseases and Sciences·Masaki KaiboriA-Hon Kwon
Mar 13, 2015·Endocrine Pathology·Jana KatuchovaPeter Kruzliak
Oct 30, 2015·Journal of Tissue Engineering and Regenerative Medicine·Sofia P RebeloPaula M Alves
Apr 26, 2013·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Dong-Liang LiJing-Jing Fan
Aug 10, 2017·Acta Cirúrgica Brasileira·Ana Karina Soares AlvesMarcelo Moura Linhares
Nov 7, 2017·Journal of Tissue Engineering and Regenerative Medicine·Aure DespeyrouxAstrid Herrero
Nov 24, 2016·Stem Cell Research & Therapy·Esteban FioreGuillermo Mazzolini

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