Stimulation of local immunity and protection in mice by intramuscular immunization with triple- or double-layered rotavirus particles and QS-21

Virology
M M McNealR L Ward

Abstract

Based on studies in animal models, parenteral immunization has become recognized as a potential vaccination strategy against rotavirus. Using an adult mouse model, the effects of the saponin adjuvant QS-21 on protection against murine rotavirus (strain EDIM) infection was determined following two intramuscular (i.m.) immunizations with purified EDIM particles including triple-layered (tl) infectious particles, tl particles inactivated with psoralen/UV, and double-layered (dl) inactivated particles. All three particles stimulated large serum rotavirus IgG responses and small amounts of serum rotavirus IgA, but undetectable stool rotavirus IgA. Inclusion of QS-21 during immunization increased the serum responses approximately 2- to 10-fold and also stimulated low levels of stool rotavirus IgA. Protection based on reduced shedding of rotavirus following EDIM challenge was significant (P < 0.001) with each immunized group and was enhanced (P < 0.001) by inclusion of QS-21 during immunization. Mice immunized with either live or inactivated tl particles and QS-21 were almost fully protected. Furthermore, animals inoculated with dl particles and the adjuvant shed significantly (P = .02) less virus following challenge than mice immuniz...Continue Reading

References

Jan 1, 1976·Archives of Virology·D R Snodgrass, P W Wells
Jul 1, 1992·Journal of Virological Methods·W S Groene, R D Shaw
Aug 1, 1991·The Journal of Infectious Diseases·D I BernsteinR L Ward
Jun 1, 1991·Journal of Virological Methods·D R KnowltonR L Ward
Jun 1, 1990·Scandinavian Journal of Immunology·E E Karagouni, L Hadjipetrou-Kourounakis
Oct 1, 1990·Journal of Virology·R L WardJ F Sheridan
Sep 1, 1988·The Pediatric Infectious Disease Journal·C ChristyR Dolin
Oct 1, 1995·European Journal of Immunology·K J MaloyA M Mowat
Nov 1, 1993·Journal of Virology·M E ConnerM K Estes
Oct 29, 1997·Journal of Virology·C M O'NealM E Conner

❮ Previous
Next ❯

Citations

Feb 13, 2008·Expert Reviews in Molecular Medicine·Richard L Ward
Jun 21, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Andrea S LaimbacherCornel Fraefel
Oct 7, 2006·Current Opinion in Gastroenterology·R D Shaw
Oct 7, 2006·Current Opinion in Gastroenterology·R D Shaw
Sep 21, 2005·Critical Reviews in Microbiology·Chi-Jen LeeXin-Xing Gu
Dec 15, 2012·Vaccine·Benoît LevastVolker Gerdts
Nov 27, 2014·Human Vaccines & Immunotherapeutics·Suvi LappalainenVesna Blazevic
Jun 20, 2013·Human Vaccines & Immunotherapeutics·Suvi LappalainenVesna Blazevic
Jul 29, 2009·Journal of Pediatric Surgery·Alexander J BondocGreg M Tiao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.