Stimulation of macrophage phagocytic but not bactericidal activity by colony-stimulating factor 1.

Infection and Immunity
C CheersE R Stanley

Abstract

The ability of mouse peritoneal cells to phagocytose and lyse Listeria monocytogenes was measured after the cells were incubated with purified murine macrophage-specific colony-stimulating factor (CSF-1). Activation of combined phagocytic and bacteriolytic ability required 24 h, with an optimal dose of 1,000 U of CSF-1 per ml. No activation was achieved with a shorter period of incubation, known to be sufficient for GM-CSF to stimulate phagocytosis by granulocytes, and there was no advantage in longer exposure. After 24 h in 1,000 U of CSF-1, macrophages showed visibly increased spreading on the plastic petri dish. Activated cells examined microscopically showed an increase in the number of phagocytic cells and in the numbers of bacteria per phagocytic cell. This increased phagocytic ability was evident also in the increase in the amount of radioactivity associated with the cells following a 30-min incubation with radiolabeled bacteria. When these cells were carefully washed, the percentage of this initial uptake released during the next 2 h was not increased by pretreatment of the cells with CSF-1, showing that the effect of this growth factor was on phagocytosis of the bacteria not on the killing mechanisms per se.

References

Jan 1, 1985·Methods in Enzymology·E R Stanley
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N A Buchmeier, R D Schreiber
Oct 1, 1984·Journal of Leukocyte Biology·R W Godfrey, M S Wilder
Jul 1, 1982·The Journal of Experimental Medicine·E A HavellP J Patel
Feb 1, 1982·The Journal of Clinical Investigation·E J WingK Stephenson

❮ Previous
Next ❯

Citations

Jan 1, 1993·Molecular Carcinogenesis·S M SchollJ W Pollard
Aug 20, 2008·Molecular and Cellular Biology·Adrian AchuthanGlen M Scholz
Nov 1, 1995·Current Eye Research·S G ElnerR M Strieter
Apr 17, 1998·Immunopharmacology and Immunotoxicology·T SakuraiK Motoyoshi
Nov 30, 2000·Current Infectious Disease Reports·D V Alcid, P Mathew
Aug 1, 1990·Journal of Cellular Physiology·W A Phillips, J A Hamilton
Dec 3, 2008·Immunology and Cell Biology·Angela TrieuMatthew J Sweet
Apr 1, 1995·Infection and Immunity·A Grieg, P Roth
Nov 1, 1993·Immunobiology·M E MielkeH Hahn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.