PMID: 6163447Feb 3, 1981Paper

Stimulation of messenger ribonucleic acid synthesis in isolated nuclei by a protein that stimulates RNA polymerase II

Biochemistry
K UenoS Natori

Abstract

A factor stimulating RNA polymerase II purified from Ehrlich ascites tumor cells was found to stimulate alpha-amanitin-sensitive RNA synthesis in nuclei isolated from spleen cells of anemic mice, though less than it stimulated purified RNA polymerase II. The fidelity of the resulting RNA synthesis was monitored by measuring the stimulation of globin mRNA synthesis. Globin mRNA was measured quantitatively by DNA-RNA hybridization by using plasmid DNA containing globin DNA sequences. Results showed that the synthesis of globin mRNA was enhanced in isolated nuclei in the presence of this factor coinciding with an increase of overall alpha-amanitin-sensitive RNA synthesis. Thus, it was concluded that an externally added factor did not stimulate random transcription but meaningful RNA synthesis in isolated nuclei.

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S Y NgR G Roeder
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G D KuehnR Braun
Jun 11, 1976·Proceedings of the National Academy of Sciences of the United States of America·T C Cheng, H H Kazazian
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S C Lee, M E Dahmus
Dec 22, 1972·Biochimica Et Biophysica Acta·M J GetzG F Saunders
Aug 28, 1973·Biochemistry·W F MarzluffR C Huang

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Citations

Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B TeyssotJ Djiane
Jul 20, 1981·FEBS Letters·Y NakanishiS Natori
May 15, 1989·Biochemical and Biophysical Research Communications·S HirashimaS Natori
Jan 1, 1982·CRC Critical Reviews in Biochemistry·I L CartwrightG C Howard

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