Stimulation of monoclonal antibody production by human-human hybridoma cells with an elevated concentration of potassium or sodium phosphate in serum-free medium.

Cytotechnology
S SatoS Hashizume

Abstract

Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1×10(5) cells/ml in dishes. At the higher cell density of 5×10(5) cells/ml, K-phosphate was more effective than Na-phosphate, at the same concentration. In large-scale continuous culture, the addition of 10 mM Na-phosphate into serum-free culture medium stimulated antibody production by HB4C5 cells 6-fold.

References

Oct 1, 1988·Journal of Surgical Oncology·T YanoK Nomoto
Jan 1, 1987·Methods in Enzymology·S HashizumeH Murakami
Oct 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H MurakamiH Omura
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H MurakamiT Kano-Sueoka

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Citations

Jan 1, 1991·Cytotechnology·O W Merten, J Litwin
Sep 8, 2009·FEMS Microbiology Letters·Perrine MarquetHarry J Flint
Jan 15, 2014·Endocrine Pathology·Esther Oliva, Robert H Young
Oct 9, 1998·The Journal of Veterinary Medical Science·S KatayamaY Shimizu

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