Stimulation of mouse cultured sympathetic neurons by uracil but not adenine nucleotides

Neuroscience
W NörenbergA U Trendelenburg

Abstract

Cultured neurons from the paravertebral sympathetic chain of rats possess excitatory P2X as well as excitatory uracil nucleotide-sensitive P2Y receptors. Preliminary observations had indicated that the analogous neurons of mice lacked P2X receptors. This difference was now investigated. Thoracolumbar sympathetic neurons from one- to three-day-old mice were cultured for seven days. When the neurons were preincubated with [3H]noradrenaline and then superfused, ATP failed to cause any change in tritium outflow. UTP (3-300 microM) and UDP (30-100 microM), in contrast, caused marked increases, and so did nicotine (3-100 microM). The effect of UTP was not changed by suramin but abolished by tetrodotoxin and in the absence of calcium. The effect of nicotine was antagonized by hexamethonium and also abolished by tetrodotoxin and in the absence of calcium. Pre-exposure to UDP prevented the effect of UTP. In neurons studied by means of whole-cell patch-clamp techniques under current clamp, ATP lacked any effect. UTP (100 microM), UDP (100 microM) and nicotine (10 microM) caused depolarization accompanied by action potentials. Pre-exposure to UDP prevented the effect of UTP. In neurons studied under voltage clamp, ATP, UTP and UDP failed ...Continue Reading

References

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Citations

May 13, 2006·Pflügers Archiv : European journal of physiology·Simon Hussl, Stefan Boehm
Apr 12, 2008·Purinergic Signalling·Beáta SperlághCecilia Csölle
May 9, 2015·Autonomic Neuroscience : Basic & Clinical·Cristina Giaroni
May 21, 2015·Purinergic Signalling·Geoffrey Burnstock, Nicholas Dale
Feb 14, 2007·The Journal of Cell Biology·Olga G ShcherbakovaBrian K Kobilka
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Apr 16, 2004·The Journal of Pharmacology and Experimental Therapeutics·Lilla PappBeáta Sperlágh

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