PMID: 9523591Apr 2, 1998Paper

Stimulation of muscarinic receptors induces expression of individual fos and jun genes through different transduction pathways

Journal of Neurochemistry
W Q DingC Alling

Abstract

The transduction pathways coupling muscarinic receptors to induction of fos and jun genes were investigated in neuroblastoma SH-SY5Y cells. Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. This effect was abolished by pretreatment with atropine, indicating an involvement of muscarinic receptors. These genes were also induced by activation of protein kinase C with phorbol ester or by elevating the intracellular Ca2+ concentration with a Ca2+ ionophore. The Ca2+ effect was inhibited by KN-62, suggesting an induction through Ca2+/calmodulin-dependent kinase II. Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately 70% but had only minor effects on the expression of c-jun and junD. On the other hand, preincubation with KN-62 attenuated the carbachol-induced increase in c-jun and junD expression by 70% but had no effect on c-fos, fosB, and junB mRNA levels. Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition...Continue Reading

Citations

Nov 17, 2001·Annals of the New York Academy of Sciences·C D Balaban
Apr 21, 1999·International Journal of Cancer. Journal International Du Cancer·R ZeidmanC Larsson
Apr 20, 2004·The European Journal of Neuroscience·Kim-Fung LinJacques Hugon

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