Stimulation of nitric oxide production in rat lung lavage cells by anti-Mac-1beta antibody: effects of ozone inhalation

American Journal of Respiratory Cell and Molecular Biology
K J PendinoD L Laskin

Abstract

Acute inhalation of the pulmonary irritant ozone is associated with an inflammatory response characterized by increased numbers of macrophages in the lung that release elevated quantities of nitric oxide. The accumulation of phagocytes in the lung is dependent on expression of leukocyte adhesion molecules including Mac-1. In the present studies, we determined whether activation of the Mac-1 receptor is involved in regulating nitric oxide production by lung phagocytes, and whether this response is modified following acute ozone inhalation. Cells were isolated from the lung by bronchoalveolar lavage 48 h after exposure of female Sprague-Dawley rats to air or ozone (2 parts per million, for 3 h). Anti-Mac-1beta antibody, but not anti-Mac-1alpha antibody, stimulated nitric oxide production by cells from both air- and ozone-exposed animals. Cells from ozone-exposed rats produced more nitric oxide and expressed greater quantities of inducible nitric oxide synthase mRNA than did cells from air-exposed animals. Production of nitric oxide in response to anti-Mac-1beta was also found to be augmented by cross-linking of the Mac-1beta receptor. Pretreatment of lavage cells with granulocyte/macrophage colony-stimulating factor (GM-CSF), whi...Continue Reading

Citations

Sep 29, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Szczepan Jozefowski, Janusz Marcinkiewicz
Dec 11, 1999·Toxicology Letters·P G ReinhartD K Bhalla
Dec 9, 1998·The American Journal of Physiology·S ProkhorovaD L Laskin
Mar 26, 1998·Biochemical and Biophysical Research Communications·R MatsunoS Tsuchiya
Nov 22, 1997·Brain Research·J L GoodwinE Uemura

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