Stimulation of nitric oxide release from rat spinal cord by prostaglandin E2

British Journal of Pharmacology
M SakaiS Ito

Abstract

1. We recently demonstrated that intrathecal administration of prostaglandin E2 (PGE2) and PGF2alpha induced allodynia through a pathway that includes the glutamate receptor and nitric oxide (NO)-generating systems from pharmacological studies. In order to clarify the involvement of NO in prostaglandin-induced allodynia, we measured NO released from rat spinal cord slices by a chemiluminescence method. 2. PGE2 stimulated NO release from both dorsal and ventral regions all along the spinal cord. PGE2 stimulated the release within 10 min and increased it in a time-dependent manner. 3. The PGE2-induced NO release was observed at 100 nM-10 microM. PGF2alpha stimulated the release at concentrations higher than 1 microM, but PGD2 (up to 10 microM) did not enhance it. 4. 17-Phenyl-omega-trinor PGE2 (EP1 > EP3) and sulprostone (EP1 < EP3) were as potent as PGE2, but PGE1 was less potent, in stimulating NO release. While M&B 28767 (EP3) did not enhance the release, butaprost (EP2) stimulated it at 1 microM. The PGE2-evoked release was blocked by ONO-NT-012, a bifunctional EP1 antagonist/EP3 agonist. 5. The PGE2-evoked release was Ca2+-dependent and blocked by MK-801 (NMDA receptor antagonist) and L-NAME (NO synthase inhibitor). The rele...Continue Reading

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Citations

Oct 2, 2002·European Journal of Pharmacology·Simon AkermanPeter J Goadsby
Aug 17, 1999·European Journal of Pharmacology·D H WrightA W Ford-Hutchinson
Mar 29, 2001·Progress in Neurobiology·H Vanegas, H G Schaible
Feb 4, 2009·Cephalalgia : an International Journal of Headache·T WieneckeM Ashina
Sep 11, 2009·Cephalalgia : an International Journal of Headache·J F TvedskovP Tfelt-Hansen
Nov 23, 2012·American Journal of Physiology. Renal Physiology·Zhanjun JiaTianxin Yang
Sep 29, 2005·Biochemical and Biophysical Research Communications·Shinji MatsumuraSeiji Ito
Jun 28, 2008·Pharmacology, Biochemistry, and Behavior·Francisca Pérez-SeverianoVinicio Granados-Soto
Sep 14, 2004·Journal of Neurobiology·Michael H OssipovFrank Porreca
Oct 25, 2008·Stroke; a Journal of Cerebral Circulation·Vikram JadhavTony Jer-Fu Lee
Aug 20, 2021·Cephalalgia : an International Journal of Headache·Maike MöllerArne May

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