Stimulation of nodulation in Medicago truncatula by low concentrations of ammonium: quantitative reverse transcription PCR analysis of selected genes

Physiologia Plantarum
Houman Fei, J K Vessey

Abstract

Although mineral nitrogen generally has negative effects on nodulation in legume-rhizobia symbioses, low concentrations of ammonium stimulate nodulation in some legumes. In this study, the effects of ammonium and nitrate on growth, nodulation and expression of 2 nitrogen transport and 12 putative nodulation-related genes of the model symbiosis of Medicago truncatula - Sinorhizobium meliloti are investigated. After 3 weeks of hydroponic growth, whole-plant nodulation was enhanced in all the ammonium treatments and up to three-fold in the 0.5 mM treatment compared with the zero-nitrogen control. Specific nodulation (nodules g(-1) root dry weight) was greatly stimulated in the 0.1 and 0.5 mM NH4+ treatments, to a lower extent in the 0.1 mM NO3- treatment, and inhibited in all other treatments. Expression of the 14 selected genes was observed at 0, 6, 12 and 24 h after exposure to rhizobia and nitrogen. Expression of nitrogen transporter genes increased significantly, but responses of the three genes putatively associated with symbiosis signaling/nodule initiation were mixed. There were infrequent responses of genes coding for an ABA-activated protein kinase or a gibberellin-regulated protein, but an ethylene-responsive element-bin...Continue Reading

References

Jan 1, 1992·The Plant Journal : for Cell and Molecular Biology·C Dehio, F J de Bruijn
Oct 1, 1994·Plant Molecular Biology·A M Hirsch, Y Fang
Oct 1, 1996·The Plant Cell·S R Long
Apr 5, 2000·Biochimica Et Biophysica Acta·S M Howitt, M K Udvardi
Jul 28, 2001·Physiologia Plantarum·Marie-Agathe LorteauFrédérique Catherine Guinel
Mar 26, 2002·Journal of Experimental Botany·Anthony D M GlassJoseph J Vidmar
Jun 1, 1995·Plant Physiology·C. CoronadoP. Ratet
Jul 1, 1995·The Plant Cell·R. A. Dixon, N. L. Paiva
Sep 18, 2004·Molecular Genetics and Genomics : MGG·E L Schnabel, J Frugoli
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Daniel Y LittleJocelyn E Malamy
Nov 1, 2005·Plant Physiology·Sam LievensMarcelle Holsters
Jun 1, 1993·Applied and Environmental Microbiology·W J Hunter
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M HirschT Bisseling
Dec 1, 1992·Plant Physiology·K H Lee, T A Larue
Jan 1, 1992·Plant Physiology·M E SamuelsonC M Larsson
Jul 15, 1988·Science·M Jacobs, P H Rubery

❮ Previous
Next ❯

Citations

Apr 15, 2014·International Journal of Molecular Sciences·Ricardo A CabezaJoachim Schulze
Aug 29, 2014·Plant Molecular Biology·Daniel StraubBenjamin Neuhäuser
Mar 31, 2012·Plant, Cell & Environment·Giuseppina CriscuoloMaurizio Chiurazzi
Sep 10, 2010·Journal of Evolutionary Biology·K D HeathJ R Stinchcombe
Feb 3, 2015·The Plant Journal : for Cell and Molecular Biology·Ricardo A CabezaJoachim Schulze
Nov 4, 2010·Plant Signaling & Behavior·Jeanne M Harris, Rebecca Dickstein
Oct 30, 2016·American Journal of Botany·Michael A GrilloKaty D Heath

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.