Stimulation of non-oxidative glucose utilization by L-carnitine in isolated myocytes

Journal of Molecular and Cellular Cardiology
S Abdel-aleemJ E Lowe

Abstract

The effects of L-carnitine on 14CO2 release from [1-14C]pyruvate oxidation (an index of pyruvate dehydrogenase activity, PDH), [2-14C]pyruvate, and [6-14C]glucose oxidation (indices of the acetyl-CoA flux through citric acid cycle), and [U-14C]glucose (an index of both PDH activity and the flux of acetyl-CoA through the citric acid cycle), were studied using isolated rat cardiac myocytes. L-carnitine increased the release of 14CO2 from [1-14C]pyruvate, and decreased that of [2-14C]pyruvate in a time and concentration-dependent manner. At a concentration of 2.5 mM, L-carnitine produced a 50% increase of CO2 release from [1-14C]pyruvate and a 50% decrease from [2-14C]pyruvate oxidation. L-carnitine also increased CO2 release from [1-14C]pyruvate oxidation by 35%, and decreased that of [2-14C]pyruvate oxidation 30%, in isolated rat heart mitochondria. The fatty acid oxidation inhibitor, etomoxir, stimulated the release of CO2 from both [1-14]pyruvate and [2-14C]pyruvate. These results were supported by the effects of L-carnitine on the CO2 release from [6-14C]- and [U-14C]glucose oxidation. L-carnitine (5 mM) decreased the CO2 release from [6-14C]glucose by 37%, while etomoxir (50 microM) increased its release by 24%. L-carnitine ...Continue Reading

Citations

Oct 17, 2012·Current Heart Failure Reports·Selim R KrimHector Ventura
Dec 23, 2003·Transfusion Medicine Reviews·Joseph D Sweeney, Arduino Arduini
Apr 8, 2003·Nutrition·Reury F P BacurauLuís F B P Costa Rosa
Dec 2, 2000·Transfusion·J D SweeneyA Arduini
Oct 27, 2011·Reproductive Toxicology·Jyotshnabala KanungoMerle G Paule
Jul 27, 2004·Journal of Clinical Apheresis·Joseph D SweeneyArduino Arduini
Nov 2, 2007·Clinical and Experimental Pharmacology & Physiology·Abdulaziz M AleisaMohamed M Sayed-Ahmed
Jul 12, 2018·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Manasa Mithun, Vani Rajashekaraiah
Oct 6, 2006·Molecular Endocrinology·Masami HiroyamaAkito Tanoue
Sep 8, 2016·American Journal of Physiology. Endocrinology and Metabolism·E Matthew MorrisJohn P Thyfault

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