Stimulation of p-nitroanisole O-demethylation in perfused livers by xylitol and sorbitol

Biochemical Pharmacology
L A ReinkeR G Thurman

Abstract

Xylitol and sorbitol, two sugar alcohols which readily enter into pathways of hepatic carbohydrate metabolism, stimulated p-nitroanisole O-demethylation in perfused livers from fasted, but not fed, phenobarbital-treated rats. The increase in mixed-function oxidation correlated well with the production of NADH from the metabolism of xylitol and sorbitol (half-maximal stimulation for both processes was observed with concentrations between 0.1 and 0.2 mM). p-Nitroanisole metabolism by isolated hepatic microsomes was unaffected by the addition of xylitol and sorbitol; however, when NADH was added to microsomes, or was generated from sorbitol, sorbitol dehydrogenase and NAD(+), a synergistic increase in p-nitroanisole metabolism occurred. Ethanol (0.2 mM), which does not enter into pathways of carbohydrate metabolism, also caused an increase in the pyridine nucleotide redox state and stimululated p-nitroanisole O-demethylation in livers from fasted rats. In addition, sorbitol and xylitol stimulated p-nitrophenol conjugation in livers from fasted, phenobarbital-treated animals, probably by supplying substrate for increased UDP-glucuronic acid synthesis. The data indicate that sugars which influence the pyridine nucleotide redox state...Continue Reading

References

Jun 14, 1957·Science·H S MASON

Citations

Jan 1, 1990·European Journal of Clinical Pharmacology·V C Ramin, J Bircher
Jan 1, 1990·Archives of Toxicology·Z Ben-ZviA Danon
Sep 1, 1986·International Journal of Biometeorology·T TakanoK Yamada
Jan 1, 1986·Alcoholism, Clinical and Experimental Research·D R Koop, M J Coon

Related Concepts

Metazoa
Liver
Nitroanisole O-Demethylase
Perfusion
Yal
Xylitol
Rats, Holtzman
Rats, Laboratory
Ethanol
Carbohydrates

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