PMID: 375988May 25, 1979Paper

Stimulation of phosphatidic acid production in platelets precedes the formation of arachidonate and parallels the release of serotonin

Biochimica Et Biophysica Acta
E G Lapetina, P Cuatrecasas

Abstract

Thrombin rapidly induces the formation of labeled phosphatidic acid from platelets prelabeled with [17C]arachidonate or 32PO34- and specifically decreases by 50--75% the content of phosphatidylinositol. Ionophore A23187 also stimulates phosphatidate labeling, but less effectively than thrombin. This effect on phosphatidic acid is blocked by increasing the levels of cyclic AMP by preincubation with dibutyryl cyclic AMP, cyclic AMP-phosphodiesterase inhibitors or prostacyclin. Indomethacin and eicosatetraynoic acid do not alter the production of phosphatidate, indicating independence from cyclooxygenase or lipoxygenase products. Increased turnover of [14C]- or [32P]phosphatidate occurs within 2--5 s after platelet activation by thrombin and is observed before endogenous, 14C-labeled arachidonate can be detected. The rate of phosphatidate formation parallels the induced rate of serotonin release. Release of [3H]serotonin is not affected by eicosatetraynoic acid. Phosphatidate production reflects the generation of diacylglycerol by C-type phospholipase degradation of phosphatidylinositol. Diacylglycerol and phosphatidic acid may participate in the membrane modification related to the early changes in platelet shape, release reactio...Continue Reading

References

Mar 25, 1975·Biochimica Et Biophysica Acta·R H Michell
Mar 1, 1977·Prostaglandins·R R GormanO V Miller
Jul 1, 1977·Prostaglandins·J M GerrardJ G White
Jun 6, 1977·Biochemical and Biophysical Research Communications·E G LapetinaP Cuatrecases
Jan 18, 1976·Biochimica Et Biophysica Acta·W C PickettP Cohen
Feb 23, 1976·Biochimica Et Biophysica Acta·T K BillsM J Silver
Feb 1, 1964·The Biochemical Journal·V P SkipskiM Barclay
Nov 1, 1972·British Journal of Haematology·J V LloydJ F Mustard
Apr 1, 1973·FEBS Letters·E G Lapetina, R H Michell

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Citations

Jun 1, 1982·Clinical Rheumatology·J P Famaey
Jan 1, 1982·Pharmacology & Therapeutics·N G Ardlie
Jan 1, 1988·Pharmacology & Therapeutics·S P Watson, P P Godfrey
Nov 1, 1987·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S IshikawaO Wada
Oct 24, 1998·Prostaglandins & Other Lipid Mediators·H JohnW Schlegel
May 23, 1998·The International Journal of Biochemistry & Cell Biology·B C KonerD Dash
Jun 19, 1980·The New England Journal of Medicine·L Viinikka, O Ylikorkala
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W SiessP Cuatrecasas
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E G LapetinaP Cuatrecasas
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J M BestermanP Cuatrecasas
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J M BestermanP Cuatrecasas
Mar 1, 1990·British Journal of Haematology·J M Cesar, J L Navarro
Sep 12, 1983·Life Sciences·E G Lapetina, W Siess
Oct 31, 1983·Biochemical and Biophysical Research Communications·F RenduS Levy-Toledano
Sep 16, 1985·Biochemical and Biophysical Research Communications·M RuggieroE G Lapetina
May 31, 1983·Biochemical and Biophysical Research Communications·L Y Chau, H H Tai
Nov 15, 1985·Biochemical and Biophysical Research Communications·S KoutouzovP Marche
Jun 13, 1986·Biochemical and Biophysical Research Communications·M A StevensonG M Hahn
Oct 29, 1987·Biochemical and Biophysical Research Communications·B N Yamaja SettyM J Stuart

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