Apr 9, 1976

Stimulation of photosystem I-induced oxidation of chloroplast cytochrome b-559 by pre-illumination and by low pH

Biochimica Et Biophysica Acta
P Horton, W A Cramer


(1) The proportion of higher plant chloroplast cytochrome b-559 oxidizable during illumination by low intensity 732 nm light increases as the pH is decreased below 6.5. At pH 5.0-5.3 total oxidation is seen and subsequent red light can cause reduction of up to 2/3 of the oxidized cytochrome. The oxidation by far red light at pH 5 is inhibited by 2 muM 2,5-dibromo-3-methyl-6-isopropyl-rho-benzoquinone whereas the red light-induced reduction is inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea. In this pH range ferricyanide-oxidized cytochrome b-559 exists in a form not reducible by ferrocyanide. (2) An increase in the amplitude of far-red induced oxidation also occurs at higher pH (up to pH 7.8) after pre-treatment of chloroplasts with substantially higher levels of light (approx. 10(6) ergs-cm-2-s-1). The degree of light activation is pH dependent, being more pronounced at lower pH. After light activation, cytochrome b-559 can be completely oxidized by far-red light in a manner reversible by red light up to pH values of 6, and the curve describing the amplitude of far-red oxidation as a function of pH is shifted by 0.5-1.0 pH unit toward higher pH. Far-red oxidation and red light reduction are again inhibited by 2,5-dibromo-...Continue Reading

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Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone

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