Stimulation of polyketide metabolism in Streptomyces fradiae by tylosin and its glycosylated precursors.

Microbiology
Steven A Fish, E Cundliffe

Abstract

Three glycosyltransferases are involved in tylosin biosynthesis in Streptomyces fradiae. The first sugar to be added to the polyketide aglycone (tylactone) is mycaminose and the gene encoding mycaminosyltransferase is orf2* (tylM2). However, targeted disruption of orf2* did not lead to the accumulation of tylactone under conditions that normally favour tylosin production; instead, the synthesis of tylactone was virtually abolished. This may, in part, have resulted from a polar effect on the expression of genes downstream of orf2*, particularly orf4* (ccr) which encodes crotonyl-CoA reductase, an enzyme that supplies 4-carbon extender units for polyketide metabolism. However, that cannot be the entire explanation, since tylosin production was restored at about 10% of the wild-type level when orf2* was re-introduced into the disrupted strain. When glycosylated precursors of tylosin were fed to the disrupted strain, they were converted to tylosin, confirming that two of the three glycosyltransferase activities associated with tylosin biosynthesis were still intact. Interestingly, however, tylactone also accumulated under such conditions and, to a much lesser extent, when tylosin was added to similar fermentations. It is concluded ...Continue Reading

References

Jun 1, 1989·Journal of Bacteriology·P MazodierC Thompson
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S E FishmanC L Hershberger
May 5, 1983·Journal of Molecular Biology·J A Gough, N E Murray
Aug 1, 1981·Antimicrobial Agents and Chemotherapy·R H Baltz, E T Seno

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Citations

Nov 11, 2009·Applied Microbiology and Biotechnology·Sung Ryeol ParkYeo Joon Yoon
May 7, 2010·Journal of Industrial Microbiology & Biotechnology·Eric Cundliffe, Arnold L Demain
Feb 28, 1992·Biochimica Et Biophysica Acta·A J ConleyJ I Mason
May 3, 2000·Molecular Microbiology·S GaisserP F Leadlay
May 3, 2002·Molecular Microbiology·Neil BateEric Cundliffe
May 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Linqi WangHuarong Tan
Jun 5, 2002·Annual Review of Biochemistry·Xuemei M He, Hung-Wen Liu
Mar 21, 2006·Research in Microbiology·Hao Jiang, C Richard Hutchinson
Apr 3, 2001·Microbiology·A R ButlerE Cundliffe
May 14, 1999·Chemistry & Biology·A R ButlerE Cundliffe
May 19, 2016·Chembiochem : a European Journal of Chemical Biology·Xiao-Long TangXin-Sheng Yao
Jun 29, 2002·Nature Biotechnology·Andrew R ButlerEric Cundliffe
Sep 1, 2006·Molecular Microbiology·Neil BateEric Cundliffe
Feb 14, 2002·Chemistry & Biology·George Stratigopoulos, Eric Cundliffe
Dec 26, 2001·Chemical Reviews·X HeH W Liu Hw

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