Stimulation of proliferation of U138-MG glioblastoma cells by gastrin-releasing peptide in combination with agents that enhance cAMP signaling

Oncology
C B FariasRafael Roesler

Abstract

Increasing evidence indicates that gastrin-releasing peptide (GRP) acts as an autocrine growth factor for brain tumors. However, it remains unclear whether the cAMP/protein kinase A (PKA) signaling pathway plays a role in mediating the mitogenic effects of GRP. We show here that GRP combined with agents that stimulate the cAMP/PKA pathway promotes proliferation of human gliobastoma cells. Treatment with GRP combined with the adenylyl cyclase activator forskolin, the cAMP analog 8-Br-cAMP or the phosphodiesterase type IV inhibitor rolipram increased proliferation of U138-MG cells in vitro measured by MTT assay. None of the compounds had an effect when given alone. GRP receptor (GRPR) mRNA and protein expression in U138-MG cells was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. The results suggest that GRP and the GRPR interact with the cAMP/PKA signaling pathway in stimulating cancer cell proliferation.

Citations

Jul 31, 2009·Journal of Molecular Neuroscience : MN·Anna Laura SchmidtRafael Roesler
Jan 23, 2013·Cytotechnology·Tiago Elias HeinenAna Beatriz Gorini da Veiga
Jan 19, 2016·Molecular Neurobiology·Pâmela Rossi MenegottoRafael Roesler
Mar 10, 2010·Brain Research Bulletin·Debora Gazzana FloresRafael Roesler
Mar 23, 2010·European Journal of Pharmacology·Gilberto L Pardo AndreuRafael Roesler
May 16, 2015·Peptides·Irene Ramos-ÁlvarezRobert T Jensen
Sep 5, 2013·Neurobiology of Learning and Memory·Rafael RoeslerZul Merali
Jul 31, 2012·Biochemical and Biophysical Research Communications·Caroline Brunetto de FariasRafael Roesler
Mar 27, 2010·Revista brasileira de psiquiatria : orgão oficial da Associação Brasileira de Psiquiatria, Asociación Psiquiátrica de la América Latina·Jaime E C HallakFlávio Kapczinski
Aug 27, 2014·Scandinavian Journal of Gastroenterology·Liping SunYuan Yuan
Jan 1, 2009·Current Opinion in Endocrinology, Diabetes, and Obesity·H Christian Weber
Aug 31, 2020·Journal of Clinical Laboratory Analysis·Wenjun LiuYulin Yuan
Jan 1, 2013·Molecular and Clinical Oncology·Daniela B CornelioRafael Roesler
Nov 18, 2010·Expert Review of Anticancer Therapy·Rafael RoeslerGilberto Schwartsmann
May 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anna Laura SchmidtRafael Roesler
Oct 10, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Jingjing ZhangXiaoyuan Chen
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Banendu Sunder DashJyh-Ping Chen

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