Stimulation of syncytium formation in vitro in human trophoblast cells by galectin-1

Placenta
Isabelle FischerUdo Jeschke

Abstract

Galectin-1 (gal-1), a member of the mammalian beta-galactoside-binding proteins, exerts biological effects by recognition of glycan ligands, including those involved in cell adhesion and growth regulation. In trophoblast cells, gal-1 binds to cell surface glycoproteins (e.g., Mucin1). It has been demonstrated that gal-1 recognizes appropriate glycotopes on the syncytiotrophoblast and extravillous trophoblast from second trimester human placenta and choriocarcinoma cells BeWo, which reveal two coexisting phenotypes, the cytotrophoblast-like and the syncytiotrophoblast-like phenotype. So the aim of this study was to investigate the effect of gal-1 on syncytium formation in BeWo and human villous trophoblasts (HVT) cells. The effect of gal-1 on syncytium formation was investigated with immunocytochemical and double immunofluorescence stainings, cell-labelling and Real-time RT-PCR. BeWo choriocarcinoma and HVT cells were incubated in vitro for 24 and 48 h in the absence (controls) and presence of gal-1 and forskolin and stained with antibodies against Ki67, beta-catenin, E-cadherin and syncytin. BeWo and HVT cells were incubated with 60 microg/ml gal-1 for 48 h (BeWo) or 96 h (HVT) and cell fusion was detected by fluorescent cell-l...Continue Reading

Citations

Jan 19, 2013·Cell Death & Disease·K WoidackiA C Zenclussen
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nancy FreitagSandra M Blois
Sep 25, 2012·Molecular Human Reproduction·Irene Tirado-GonzálezSandra M Blois
Oct 1, 2013·Human Reproduction Update·Gabriela BarrientosSandra M Blois
Mar 19, 2014·Molecular Human Reproduction·Sandra M BloisSilvia Daher
Sep 23, 2014·Current Hypertension Reports·Anjali AcharyaTanvi Rege
Jun 3, 2011·Journal of Reproductive Immunology·Isabelle FischerAlexander G Betz
Mar 10, 2016·Journal of Reproductive Immunology·Annegret SchnabelBirgit Seelbach-Göbel
Jan 28, 2015·Journal of Reproductive Immunology·Sandra M BloisGabriela Barrientos
Sep 6, 2014·Frontiers in Immunology·Nándor Gábor ThanHamutal Meiri
Sep 7, 2014·Cellular and Molecular Life Sciences : CMLS·Sandra M BloisAnne Cathrine Staff
May 3, 2016·International Journal of Molecular Sciences·Laura UnverdorbenStefan Hutter
Mar 2, 2016·Molecular & Cellular Proteomics : MCP·Qiushi ChenGary F Clark
Feb 22, 2012·Reproductive Sciences·Amandine VargasBenoit Barbeau
Sep 10, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Caroline ToudicBenoit Barbeau
Jun 25, 2019·Frontiers in Immunology·Sandra M BloisGabriela Barrientos

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