Abstract
In addition to the airway-relaxing effects, β2 adrenergic receptor (β2AR) agonists are also found to have broad anti-inflammatory effects. The current study was conducted to define the role of β2AR agonists in limiting myocardial ischemia/reperfusion injury (IRI). Methods and Results: Adult male wild-type (WT) and interleukin (IL)-10 knockout (KO) mice underwent a 40-min left coronary artery ligation and 60-min reperfusion. A selective β2AR agonist, Clenbuterol, at doses of 0.1 μg or 1 μg/g weight i.v. 5 min before reperfusion, significantly reduced myocardial infarct size (IS) by 28% and 39% (vs. control, P<0.05) in WT mice respectively, but had no protective effect in IL-10 KO mice. Inhalational therapy with nebulized Clenbuterol, Albuterol, Salmeterol or Arformoterol immediately before ischemia significantly reduced IS (P<0.05) in WT mice. Splenectomy similarly reduced IS as Clenbuterol-treated mice, but intravenous Clenbuterol did not further reduce IS in splenectomized mice. In splenectomized WT mice, acute transfer of isolated splenocytes, not the Clenbuterol-pretreated splenocytes, restored the myocardial IS to the level of intact mice. Intravenous Clenbuterol significantly increased splenic protein levels of β2AR, phosp...Continue Reading
References
Dec 1, 1993·Pharmacology & Therapeutics·O E Brodde
Mar 1, 1993·Journal of Cardiac Surgery·R F Appleyard, L H Cohn
Apr 22, 1996·Brain Research·I SemkovaJ Krieglstein
Apr 1, 1997·The American Journal of Physiology·P G BloemenF P Nijkamp
Oct 27, 2004·Anesthesia and Analgesia·Torsten LoopBenedikt H J Pannen
Apr 27, 2005·Circulation·Zequan YangJoel Linden
Nov 8, 2005·International Archives of Allergy and Immunology·Kozo YasuiAtsushi Baba
Oct 25, 2006·Circulation·Zequan YangJoel Linden
Feb 19, 2008·British Journal of Clinical Pharmacology·Frank de VriesTjeerd van Staa
May 27, 2008·Acta Pharmacologica Sinica·Ping LiuQin Fu
Oct 14, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Zequan YangBrent A French
Nov 17, 2009·International Journal of Cardiology·Borja IbanezJuan J Badimon
Jul 2, 2010·British Journal of Pharmacology·Qiufang ZhangQin Fu
May 13, 2011·Cardiovascular Drugs and Therapy·Ming-He HuangKenichi Fujise
Aug 4, 2011·Journal of Biomedical Science·Yikui TianMinxin Wei
Aug 23, 2011·Brain, Behavior, and Immunity·Virginia M Sanders
Feb 10, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Markus BosmannPeter A Ward
Jun 2, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Shashi BhushanDavid J Lefer
Dec 29, 2013·Oxidative Medicine and Cellular Longevity·Zequan YangBrent A French
Jan 7, 2014·PloS One·Binbin ChenJunyan Yao
Feb 11, 2014·Journal of the American Society of Nephrology : JASN·Sean R JesinkeyRick G Schnellmann
Mar 20, 2014·Journal of Innate Immunity·Jamison J GrailerPeter A Ward
Apr 29, 2014·American Heart Journal·Kevin R Bainey, Paul W Armstrong
May 28, 2015·Basic Research in Cardiology·Yikui TianZequan Yang
Sep 20, 2015·European Heart Journal. Acute Cardiovascular Care·Rasmus RørthLars Køber
Dec 10, 2015·European Heart Journal·Ulrich Hofmann, Stefan Frantz
Aug 4, 2016·Brain, Behavior, and Immunity·Donald M LamkinErica K Sloan
Sep 21, 2016·Basic Research in Cardiology·Yikui TianZequan Yang
Oct 25, 2016·Journal of Molecular and Cellular Cardiology·Denise MathesUlrich Hofmann
Feb 7, 2017·Cytokine·Tiina KeränenRiku Korhonen
Apr 12, 2017·Kidney International·Hyunjin NohGeorge L King
Apr 19, 2017·Heart·Robert D BrookUNKNOWN SUMMIT Investigators
Citations
Jan 4, 2019·Circulation Research·Gerd Heusch
Mar 3, 2020·Circulation Journal : Official Journal of the Japanese Circulation Society·YanHua WangYiDeng Jiang
Dec 7, 2020·The Journal of Surgical Research·Aimee ZhangZequan Yang
Dec 17, 2019·The Journal of Thoracic and Cardiovascular Surgery·Eric J CharlesZequan Yang
May 1, 2021·Cells·Anne-Sophie Colombe, Guillaume Pidoux
May 18, 2021·Frontiers in Cardiovascular Medicine·Niek J PluijmertPaul H A Quax
Aug 28, 2021·Micromachines·Arsalan Ul HaqFabio De Matteis