PMID: 2507320Sep 15, 1989Paper

Stimulation of the methylcoenzyme M reduction by uridine-5'-diphospho-sugars in cell-free extracts of Methanobacterium thermoautotrophicum (strain delta H)

European Journal of Biochemistry
J T KeltjensG D Vogels

Abstract

The hydrogen-dependent reduction of methylcoenzyme M catalyzed by coenzyme-depleted cell-free extracts of Methanobacterium thermoautotrophicum was stimulated by micromolar concentrations of a UDP-disaccharide present in the organism. The compound was isolated and identified as UDP-1-O-alpha-D-2-acetamido-2-deoxyglucopyranose (UDPGlcpNAc) glycosidically linked to 2-acetamido-2-deoxymannopyranosyluronic acid. Maximal stimulation was observed when both the UDP-disaccharide and mercaptoheptanoylthreonine phosphate were present in the reaction mixtures. The UDP derivative isolated was not specific in its action: other UDP-sugars tested in micromolar concentrations stimulated the methylcoenzyme M reduction to the same extent. The activated sugars presumably substitute for ATP, which is usually required in much higher concentrations to activate the methylcoenzyme M reductase system.

References

May 1, 1977·Analytical Biochemistry·J J Sedmak, S E Grossberg
Mar 31, 1989·Biochemical and Biophysical Research Communications·B J MarsdenJ K Kramer
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K M NollR S Wolfe
Sep 30, 1986·Biochemical and Biophysical Research Communications·K M Noll, R S Wolfe
Sep 30, 1987·Biochemical and Biophysical Research Communications·F D SauerJ K Kramer
Oct 1, 1985·Journal of Bacteriology·W B Whitman, R S Wolfe
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D P Nagle, R S Wolfe

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Citations

Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·J G Ferry
May 15, 1991·FEMS Microbiology Letters·W M van de WijngaardC van der Drift
Feb 1, 1990·Journal of Bacteriology·P C Raemakers-FrankenG D Vogels

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