Stimulation of the proximal tubule Na+-ATPase activity by adenosine A(2A) receptor

The International Journal of Biochemistry & Cell Biology
M WengertCelso Caruso-Neves

Abstract

The aim of this work was to determine the molecular mechanism involved in the stimulation of the pig kidney proximal tubule Na+-ATPase by adenosine (Ado). To study the role of A2 Ado receptors, we added in all experiments 10(-6)M DPCPX, an A1 receptor-selective antagonist, since we have previously shown that Ado inhibits the enzyme activity through this receptor. Ado increased the Na+-ATPase activity with maximal effect observed at 10(-6)M. The presence of both A(2A) and A(2B) receptors were demonstrated by immunoblotting using specific polyclonal antibodies. The stimulatory effect of Ado was completely abolished by 5 x 10(-9)M DMPX, an antagonist of A2 receptor, and 10(-7)M SCH 58261, an A(2A) receptor-selective antagonist. DMPA (10(-7)M), a specific agonist of A(2A) receptor mimicked the stimulatory effect of Ado. Involvement of a Gs protein/adenylate cyclase/PKA pathway was evidenced by: (a) the reversion of Ado-induced effect by GDPbetaS; (b) stimulation of the Na+-ATPase activity in a similar and non-additive manner to Ado by 10(-8)M cholera toxin, 10(-7)M GTPgammaS, 10(-6)M forskolin, 10(-7)M cAMP or 1.25 U catalytic subunit of PKA; (c) the reversion of the stimulatory effect of Ado by 10(-8)M PKA inhibitor peptide; (d) A...Continue Reading

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Citations

Jul 29, 2008·Current Opinion in Nephrology and Hypertension·Francesca Di Sole
Nov 23, 2013·Purinergic Signalling·Geoffrey BurnstockMatthew A Bailey
May 1, 2012·Pflügers Archiv : European journal of physiology·Miguel A RocafullJesús R del Castillo
Sep 16, 2016·Journal of the American Society of Nephrology : JASN·Sun-Sang J SungMark D Okusa
Oct 8, 2014·Acta Physiologica·M M Rabadi, H T Lee
Aug 28, 2009·Archives of Biochemistry and Biophysics·Natália Assaife-LopesCelso Caruso-Neves

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