Stimulation of TRPC5 cationic channels by low micromolar concentrations of lead ions (Pb2+).

Biochemical and Biophysical Research Communications
Piruthivi Sukumar, D J Beech

Abstract

Lead toxicity is long-recognised but continues to be a major public health problem. Its effects are wide-ranging and include induction of hyper-anxiety states. In general it is thought to act by interfering with Ca(2+) signalling but specific targets are not clearly identified. Transient receptor potential canonical 5 (TRPC5) is a Ca(2+)-permeable ion channel that is linked positively to innate fear responses and unusual amongst ion channels in being stimulated by trivalent lanthanides, which include gadolinium. Here we show investigation of the effect of lead, which is a divalent ion (Pb(2+)). Intracellular Ca(2+) and whole-cell patch-clamp recordings were performed on HEK 293 cells conditionally over-expressing TRPC5 or other TRP channels. Extracellular application of Pb(2+) stimulated TRPC5 at concentrations greater than 1 microM. Control cells without TRPC5 showed little or no response to Pb(2+) and expression of other TRP channels (TRPM2 or TRPM3) revealed partial inhibition by 10 microM Pb(2+). The stimulatory effect on TRPC5 depended on an extracellular residue (E543) near the ion pore: similar to gadolinium action, E543Q TRPC5 was resistant to Pb(2+) but showed normal stimulation by the receptor agonist sphingosine-1-ph...Continue Reading

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Citations

Oct 15, 2013·British Journal of Pharmacology·Julia M RichterKerstin Hill
Aug 12, 2014·Pflügers Archiv : European journal of physiology·Alexandre BouronJohannes Oberwinkler
Mar 23, 2017·The Journal of Biological Chemistry·Hussein N RubaiyDavid J Beech
Mar 22, 2014·Chemistry : a European Journal·James Guo Sheng MooMartin Pumera
Dec 4, 2010·The Journal of Biological Chemistry·Jacqueline NaylorDavid J Beech
May 14, 2019·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·SeungJoo JeongInsuk So
Jan 20, 2016·The Journal of Veterinary Medical Science·Masato OhkuboMitsuya Shiraishi
Apr 26, 2016·Metallomics : Integrated Biometal Science·Rakshya GorkhaliJenny J Yang

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