Stimulation of V(D)J cleavage by high mobility group proteins

The EMBO Journal
D C van GentM Gellert

Abstract

V(D)J recombination requires a pair of signal sequences with spacer lengths of 12 and 23 bp between the conserved heptamer and nonamer elements. The RAG1 and RAG2 proteins initiate the reaction by making double-strand DNA breaks at both signals, and must thus be able to operate on these two different spatial arrangements. We show that the DNA-bending proteins HMG1 and HMG2 stimulate cleavage and RAG protein binding at the 23 bp spacer signal. These findings suggest that DNA bending is important for bridging the longer spacer, and explain how a similar array of RAG proteins could accommodate a signal with either a 12 or a 23 bp spacer. An additional effect of HMG proteins is to stimulate coupled cleavage greatly when both signal sequences are present, suggesting that these proteins also aid the formation of a synaptic complex.

References

Jan 1, 1992·Annual Review of Genetics·M Gellert
Oct 1, 1995·Genes & Development·D A Ramsden, M Gellert
Jun 16, 1995·Cell·D C van GentM Gellert
Jun 1, 1995·Genes & Development·B M ShykindP A Sharp
Mar 1, 1994·Trends in Genetics : TIG·R GrosschedlJ Pagel
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P M PilS J Lippard
Jun 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S B SteenD B Roth

❮ Previous
Next ❯

Citations

May 27, 2005·DNA Repair·Raymond Reeves, Jennifer E Adair
Nov 7, 2002·Journal of Molecular Biology·Ulrich Rass, Börries Kemper
May 29, 1998·Current Opinion in Immunology·U GrawunderM R Lieber
Feb 19, 2000·Current Opinion in Structural Biology·A Travers
Mar 14, 2001·Trends in Biochemical Sciences·J O Thomas, A A Travers
Mar 25, 2005·Nature Reviews. Drug Discovery·Dong Wang, Stephen J Lippard
Apr 28, 2009·Nature Structural & Molecular Biology·Fang Fang YinDavid G Schatz
Jul 28, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N YamashitaO Koiwai
Sep 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Wilfred NdifonNir Friedman
Jun 9, 2009·The Journal of Biological Chemistry·Luda S ShlyakhtenkoYuri L Lyubchenko
Jul 30, 2002·The EMBO Journal·Jessica M Jones, Martin Gellert
Oct 23, 1997·Nucleic Acids Research·Q M Eastman, D G Schatz
Jul 25, 2000·Nucleic Acids Research·L A Hanakahi, N Maizels
Mar 27, 2001·Nucleic Acids Research·M J Sadofsky
Apr 25, 2003·Nucleic Acids Research·Hatem ZayedZoltán Ivics
Feb 19, 2008·Nucleic Acids Research·Prafulla RavalPatrick C Swanson
Jan 8, 2013·Nucleic Acids Research·Mihai CiubotaruDavid G Schatz
Jan 18, 2013·Nucleic Acids Research·Alicia J LittleDavid G Schatz
Aug 15, 1997·Genes & Development·E L Beall, D C Rio
Mar 21, 1998·Genes & Development·L JayaramanC Prives
Jun 2, 2006·Molecular and Cellular Biology·Aleksei N KriatchkoPatrick C Swanson
May 29, 2000·Molecular and Cellular Biology·K B EllwoodM Carey
Jan 3, 2001·Molecular and Cellular Biology·S B KaleD B Roth
Mar 10, 2001·Molecular and Cellular Biology·X MoM J Sadofsky
Dec 12, 2001·Molecular and Cellular Biology·Cynthia L MundyMarjorie A Oettinger
Sep 21, 2002·Molecular and Cellular Biology·Fumikiyo NagawaHitoshi Sakano
Feb 13, 2002·Molecular and Cellular Biology·Patrick C Swanson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.