PMID: 8584007Oct 4, 1995Paper

Stimulatory effect of hormonal signaling factors on (Ca(2+)-Mg2+)-ATPase activity in rat liver plasma membranes: cross talk with regucalcin

Molecular and Cellular Biochemistry
H TakahashiM Yamaguchi

Abstract

The effect of hormonal signaling factors on (Ca(2+)-Mg2+)-ATPase activity in rat liver plasma membranes was investigated. The presence of inositol-glycan (10(-7)-10(-5) M), dibutyryl cAMP (10(-4) and 10(-3) M) or inositol 1,4,5-trisphosphate (IP3; 10(-6) and 10(-5) M) in the enzyme reaction mixture produced a significant increase in (Ca(2+)-Mg2+)-ATPase activity. These effects were completely inhibited by the presence of vanadate (10(-4) M), an inhibitor of the enzyme phosphorylation, and N-ethylmaleimide (5 x 10(-3) M), a SH group modifying reagent. Meanwhile, regucalcin, a Ca(2+)-binding protein isolated from rat liver cytosol, increased the enzyme activity by binding to the SH groups of (Ca(2+)-Mg2+)-ATPase in liver plasma membranes. The presence of regucalcin (0.25 microM) with an effective concentration completely inhibited the effect of inositol-glycan (10(-5) M) to increase (Ca(2+)-Mg2+)-ATPase activity, while the effect of dibutyryl cAMP (10(-3) M) or IP3 (10(-5) M) was not altered. The inositol-glycan effect was not modulated by the presence of dibutyryl cAMP or IP3. Now, the preincubation of the plasma membranes with regucalcin did not modify the effect of inositol-glycan on the enzyme activity, suggesting that reguca...Continue Reading

References

Jun 1, 1978·Chemical & Pharmaceutical Bulletin·M Yamaguchi, T Yamamoto
Jun 1, 1984·The Biochemical Journal·M J Berridge
Nov 1, 1984·Biological Reviews of the Cambridge Philosophical Society·P H ReinhartF L Bygrave
Dec 30, 1981·Biochimica Et Biophysica Acta·J R WilliamsonJ B Hoek
Aug 25, 1993·Molecular and Cellular Biochemistry·H Takahashi, M Yamaguchi

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Citations

Jun 5, 1998·The International Journal of Biochemistry & Cell Biology·D R Jones, I Varela-Nieto
Mar 29, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Chamilani NikapitiyaJehee Lee
Mar 25, 2011·Molecular and Cellular Biochemistry·Masayoshi Yamaguchi
May 16, 2000·Life Sciences·M Yamaguchi

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