Stimulatory Effect of Magnetite Nanoparticles on a Highly Enriched Butyrate-Oxidizing Consortium

Frontiers in Microbiology
Li FuYahai Lu

Abstract

Syntrophic oxidation of butyrate is catabolized by a few bacteria specialists in the presence of methanogens. In the present study, a highly enriched butyrate-oxidizing consortium was obtained from a wetland sediment in Tibetan Plateau. During continuous transfers of the enrichment, the addition of magnetite nanoparticles (nanoFe3O4) consistently enhanced butyrate oxidation and CH4 production. Molecular analysis revealed that all bacterial sequences from the consortium belonged to Syntrophomonas with the closest relative of Syntrophomonas wolfei and 96% of the archaeal sequences were related to Methanobacteria with the remaining sequences to Methanocella. Addition of graphite and carbon nanotubes for a replacement of nanoFe3O4 caused the similar stimulatory effect. Silica coating of nanoFe3O4 surface, however, completely eliminated the stimulatory effect. The control experiment with axenic cultivation of a Syntrophomonas strain and two methanogen strains showed no effect by nanoFe3O4. Together, the results in the present study support that syntrophic oxidation of butyrate is likely facilitated by direct interspecies electron transfer in the presence of conductive nanomaterials.

References

Jan 1, 1967·Archiv für Mikrobiologie·M P BryantR S Wolfe
Jan 4, 2001·FEMS Microbiology Ecology·K L StraubB Schink
Mar 18, 2008·Applied and Environmental Microbiology·Jingjing PengYahai Lu
Jul 1, 2008·Nature Reviews. Microbiology·Rudolf K ThauerReiner Hedderich
May 26, 2009·Applied and Environmental Microbiology·Junpeng RuiYahai Lu
Jul 18, 2009·Nature Reviews. Microbiology·Alfons J M Stams, Caroline M Plugge
Nov 10, 2009·Current Opinion in Biotechnology·Michael J McInerneyRobert P Gunsalus
Apr 5, 2011·Applied and Environmental Microbiology·Pengfei LiuYahai Lu
Aug 1, 2010·Environmental Microbiology·Jessica R SieberMichael J McInerney
Oct 19, 2011·Environmental Microbiology·Souichiro KatoKazuya Watanabe
May 15, 2012·Applied and Environmental Microbiology·Yanlu GanYahai Lu
Jun 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Souichiro KatoKazuya Watanabe
Jun 29, 2012·The ISME Journal·Christopher B WalkerDavid A Stahl
Jul 19, 2012·Annual Review of Microbiology·Jessica R SieberRobert P Gunsalus
Oct 1, 2009·Environmental Microbiology Reports·Harold L DrakePia K Wüst
Oct 1, 2009·Environmental Microbiology Reports·Quan Yuan, Yahai Lu
Jan 7, 2014·Environmental Microbiology·Jessica R SieberMichael J McInerney
May 23, 2014·Scientific Reports·Shanshan ChenDerek R Lovley
Jun 6, 2014·Environmental Science & Technology·Carolina Cruz ViggiFederico Aulenta
Jun 29, 2014·Biochimica Et Biophysica Acta·Petra WormAlfons J M Stams
Oct 7, 2014·Bioresource Technology·Shanshan ChenDerek R Lovley
Nov 12, 2014·Environmental Microbiology·Derek R Lovley, Nikhil S Malvankar
Mar 5, 2015·MBio·Nikhil S MalvankarDerek R Lovley
Aug 19, 2015·Frontiers in Microbiology·Amelia-Elena RotaruDerek R Lovley
Sep 17, 2015·Nature·Shawn E McGlynnVictoria J Orphan
Dec 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Mallory EmbreeKarsten Zengler
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Feb 17, 2017·Environmental Microbiology Reports·Bernhard SchinkNicolai Müller
Apr 28, 2017·Environmental Microbiology·Andreia F SalvadorM Madalena Alves

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Datasets Mentioned

BETA
KT203965

Methods Mentioned

BETA
PCR
density gradient fractionation

Software Mentioned

MEGA7
SPSS
DIET

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