PMID: 7009287Jan 1, 1981

Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture

J H NielsenA Andersson


Islets of Langerhans from NMRI-mice were kept for one week in tissue culture in medium supplemented with human serum obtained from either normal healthy subjects or newly diagnosed juvenile diabetic patients before insulin treatment. Islets cultured in diabetic serum released more insulin than islets cultured in normal serum, whether tissue culture medium 199 with 5.5-8.3 mmol/l glucose and 10% serum, or culture medium RPMI 1640 with 11 mmol/l glucose and 0.5% serum were used. Islets kept for one week in culture with diabetic serum did not show any decrease in DNA content or glucose induced insulin secretion and biosynthesis. It is concluded that serum from newly diagnosed insulin-dependent diabetic patients stimulates insulin release from isolated mouse islets kept in tissue culture. The underlying mechanism is unknown.


Dec 1, 1992·Journal of Pharmacological and Toxicological Methods·S el RaziA R Carpinelli
Jun 11, 1981·The New England Journal of Medicine·G F Cahill, H O McDevitt


Jan 1, 1977·Annual Review of Physiology·L R Johnson
Jan 1, 1972·Diabetes·A Andersson, C Hellerström
Jan 23, 1971·British Medical Journal·P ZimmetH P Taft
Jul 1, 1963·The Biochemical Journal·C N HALES, P J RANDLE

Related Concepts

Magnetic Resonance Imaging
NMRI Mouse
Culture Techniques
Glucose, (L)-Isomer
Insulin Secretion
Endocrine Pancreas - Islet of Langerhans (Mmhcc)
Islets of Langerhans
Tissue Culture

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