PMID: 9171412Jun 1, 1997Paper

Stimulatory effect of trehalose on formation and activity of Escherichia coli RNA polymerase E sigma38 holoenzyme

Journal of Bacteriology
S Kusano, A Ishihama

Abstract

The intracellular concentration of trehalose increases in the stationary-phase cells of Escherichia coli. The effects of trehalose on transcription in vitro by E. coli RNA polymerase were compared for two holoenzymes, E sigma70 and E sigma38, which were reconstituted from purified core enzyme and either sigma70 (the major sigma at the exponential growth phase) or sigma38 (the essential sigma at the stationary growth phase), respectively. The optimum trehalose concentration giving maximum transcription by E sigma38 was higher than that by E sigma70. Transcription activation by trehalose was attributed to both increased formation of E sigma38 holoenzyme and increased transcription initiation by E sigma38 from sigma38-dependent promoters. The activation of E sigma38 by trehalose was additive with the transcription enhancement by decreased superhelicity of template DNA prepared from stationary-phase cells. We thus propose that the selective activation of transcription by E sigma38 holoenzyme takes place in the presence of specific conditions and factors present under stress conditions.

References

Nov 1, 1991·Molecular & General Genetics : MGG·M OzakiA Ishihama
Jan 1, 1988·Annual Review of Biochemistry·J D Helmann, M J Chamberlin
Oct 1, 1988·Trends in Genetics : TIG·A Ishihama
Jan 1, 1982·Annual Review of Genetics·B Magasanik
Jan 1, 1984·Annual Review of Genetics·F C NeidhardtV Vaughn
Jan 1, 1993·Annual Review of Microbiology·R KolterA Tormo

❮ Previous
Next ❯

Citations

Oct 10, 1998·Molecular Microbiology·A FarewellT Nyström
Sep 28, 2002·The Journal of Biological Chemistry·Shun Jin Lee, Jay D Gralla
Oct 6, 2000·Annual Review of Microbiology·A Ishihama
Feb 17, 2007·Molecular Microbiology·Athanasios TypasRegine Hengge
Dec 12, 2001·Molecular Microbiology·T GaalR L Gourse
Mar 25, 2006·Molecular Microbiology·Jorge M SantosCecília M Arraiano
May 29, 2001·The Journal of Biological Chemistry·S J Lee, J D Gralla
Sep 15, 2017·The Journal of General and Applied Microbiology·Akira Ishihama
Aug 19, 2008·Applied and Environmental Microbiology·Manfredo J SeufferheldMaria E Farias
Dec 6, 1997·Current Opinion in Genetics & Development·A Ishihama
Jan 27, 1999·Mutation Research·A Eisenstark
Apr 22, 2004·Molecular Cell·Shun Jin Lee, Jay D Gralla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.