PMID: 8959758Jan 1, 1996Paper

Stimulatory effect of zinc-chelating dipeptide on deoxyribonucleic acid synthesis in osteoblastic MC3T3-E1 cells

Peptides
M Yamaguchi, T Matsui

Abstract

Whether deoxyribonucleic acid (DNA) synthesis in osteoblastic MC3T3-E1 cells is stimulated by zinc, an activator of bone formation, was investigated in vitro. After subculture for 3 days, the cells were cultured for up to 3 days (72 h) with zinc sulfate or zinc-chelated dipeptide (beta-alanyl-L-histidinato zinc; AHZ) in the range of 10(-7) to 10(-5) M. The culture with zinc compounds (10(-5) M) produced a significant increase of cell number, DNA content, and protein concentration in the cells, as reported previously. The culture with zinc compounds (10(-6) and 10(-5) M) clearly stimulated DNA synthesis in the homogenate, when it was estimated by the incorporation of [3H]deoxythymidine 5'-triphosphate into the DNA in the homogenate of cells. The AHZ effect was greater than that of zinc sulfate. The culture together with cycloheximide (19(-6) M) completely abolished the zinc compounds (10(-5) M)-induced increase of DNA synthesis in the cells, suggesting that the zinc compound effect is based on a newly synthesized protein component. Moreover, when zinc sulfate (10(-7) and 10(-6) M) or AHZ (10(-8) to 10(-5) M) was added into the reaction mixture with the homogenate of cells cultured without zinc compounds, the DNA synthesis was cl...Continue Reading

References

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Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T K GrayL M Nabell
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Oct 1, 1995·General Pharmacology·M Yamaguchi

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Citations

Jul 20, 2007·Biological Trace Element Research·Aleksandra CerovicAhmed El-Sohemy
Sep 17, 2005·Journal of Materials Science. Materials in Medicine·D Boyd, M R Towler
Aug 1, 2007·Journal of Materials Science. Materials in Medicine·Daniel BoydOwen M Clarkin
May 12, 2006·Journal of Materials Science. Materials in Medicine·D BoydR G Hill
Jan 27, 2009·Journal of Endodontics·Chia-Tze KaoShinn-Jyh Ding
Mar 23, 2007·Journal of Biomedical Materials Research. Part a·Jenni R PoppAaron S Goldstein
Jan 18, 2011·Journal of Endodontics·Chia-Yung LinMin-Huey Chen
Jun 9, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Fan YangGuo-li Yang
Apr 6, 2012·Molecular and Cellular Biochemistry·Masayoshi Yamaguchi
Mar 11, 2008·Molecular and Cellular Biochemistry·Masayoshi YamaguchiTaeko Nakagawa
Dec 26, 2009·Molecular and Cellular Biochemistry·Masayoshi Yamaguchi
Oct 1, 2012·Materials Science & Engineering. C, Materials for Biological Applications·Gurbinder KaurK Singh

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