Stimulatory effects of NESFATIN-1 on meiotic and developmental competence of porcine oocytes

Journal of Cellular Physiology
Zubing CaoYunhai Zhang

Abstract

NESFATIN-1 acts as a neuroendocrine hormone to suppress gonadotropin secretion in the female goldfish and to prevent germinal vesicle breakdown of oocytes in the zebrafish. However, the expression and function of NESFATIN-1 in meiotic maturation and development of porcine oocytes remains elusive. Genomic structure of porcine NESFATIN-1 precursor nucleobindin 2 (NUCB2) is first characterized in detail and an evolutionally closer relationship of NESFATIN-1 between pig and rat is shown by phylogenetic analysis of multiple species. Additionally, immunofluorescence analysis revealed that NESFATIN-1 is predominantly expressed and localizes on the membrane of both theca cells and granulosa cells, but not expressed in oocytes. Real-time quantitative polymerase chain reaction showed that the abundance of NESFATIN-1 transcripts in granulosa cells progressively decreases during the developmental transition from small follicles to large follicles. Correspondingly, NESFATIN-1 could significantly enhance both the cleavage and blastocyst rate of parthenogenetically activated oocytes from small follicles (p < 0.05), whereas it did not affect meiotic maturation and development of oocytes from large follicles. Interestingly, we found that NESFAT...Continue Reading

References

May 1, 1985·The Journal of Experimental Zoology·H J Leese, A M Barton
Apr 5, 2002·Molecular Reproduction and Development·Stephen M DownsHenry J Leese
Jun 4, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·H Gonzales-Figueroa, H M Gonzales-Molfino
Oct 13, 2006·Nature·Shinsuke Oh-IMasatomo Mori
Jul 12, 2008·Molecular Reproduction and Development·Sarah E HarrisHelen M Picton
Jun 11, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David García-GalianoManuel Tena-Sempere
May 20, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ashton E LehmannPhu V Tran
Jun 15, 2011·Molecular Reproduction and Development·Jeffrey J Whyte, Randall S Prather
Aug 17, 2012·Biology of Reproduction·Ronald GonzalezSuraj Unniappan
Feb 19, 2013·The International Journal of Developmental Biology·Esther Collado-FernandezRémi Dumollard
Mar 26, 2013·Endocrine·Suleyman Aydin
Mar 30, 2013·Current Pharmaceutical Design·David García-Galiano, Manuel Tena-Sempere
Mar 30, 2013·Current Pharmaceutical Design·Haneesha Mohan, Suraj Unniappan
Jun 4, 2014·Reproduction in Domestic Animals = Zuchthygiene·E DovolouG S Amiridis
May 11, 2016·Theriogenology·Darryl L RussellJeremy G Thompson
Sep 3, 2016·Current Opinion in Pharmacology·Philip Prinz, Andreas Stengel
Sep 8, 2016·Scientific Reports·Xiaoxiao GaoYa Liu
Oct 19, 2016·The Journal of Endocrinology·Riccardo DoreCarla Schulz
Apr 6, 2017·Molecular Reproduction and Development·Debabrata Das, Swathi Arur
Apr 11, 2017·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Miro KasumEmina Ejubović
Feb 13, 2018·Human Reproduction Update·Marco Conti, Federica Franciosi
Jun 18, 2018·Domestic Animal Endocrinology·K A MortonS Unniappan
Sep 21, 2018·Frontiers in Genetics·Huaqiang Yang, Zhenfang Wu
Oct 12, 2018·Journal of the Endocrine Society·Martha A Schalla, Andreas Stengel

❮ Previous
Next ❯

Citations

Aug 1, 2021·General and Comparative Endocrinology·Krittika DotaniaUmesh Rai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.