PMID: 9187267Jun 1, 1997Paper

Stimulatory roles of muscarinic acetylcholine receptors on T cell antigen receptor/CD3 complex-mediated interleukin-2 production in human peripheral blood lymphocytes

Molecular Pharmacology
H FujinoY Nomura

Abstract

It is known that there are some bidirectional interactions between the nervous and the immune systems via neurotransmitters and cytokines. To clarify whether any neurotransmitters modulate lymphocyte functions, we examined the effects of oxotremorine-M (Oxo-M) on interleukin-2 (IL-2) production in human peripheral blood lymphocytes by using enzyme-linked immunosorbent assays, Northern blot analyses, reverse transcriptase-polymerase chain reaction, and fluorescence-activated cell sorter. Pretreatment of cells with Oxo-M (10 nM to 10 microM) for 4-24 hr enhanced phytohemagglutinin (PHA)-induced IL-2 mRNA expression and markedly increased IL-2 production compared with those induced by PHA alone. Oxo-M alone did not affect IL-2 mRNA expression and IL-2 production. In CD3-positive T cells, pretreatment with Oxo-M for 24 hr enhanced PHA-induced IL-2 production. Furthermore, pretreatment with Oxo-M enhanced PHA-induced mRNA expression of the alpha and beta subunits of IL-2 receptors and DNA synthesis. Cytometric analysis showed Oxo-M treatment did not up-regulate expression of cell surface molecules such as CD3, CD2, CD4, CD8, and IL-2 receptors. These results suggest that activation of muscarinic receptors enhances T cell antigen rec...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H M ShapiroL A Kamentsky
Apr 1, 1992·Immunology Today·S L Schreiber, G R Crabtree
Jul 1, 1992·Immunology Today·A Arnaiz-VillenaJ R Regueiro
Oct 1, 1992·Trends in Biochemical Sciences·Y AsaokaY Nishizuka
Oct 1, 1992·Trends in Biochemical Sciences·R T AbrahamP J Leibson
Mar 1, 1992·Immunology Today·J DownwardD Cantrell
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T J Novak, E V Rothenberg
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J C ReedR G Hoover
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H M Shapiro, T B Strom
Apr 9, 1993·Cell·T Taniguchi, Y Minami
Apr 16, 1993·Brain Research. Developmental Brain Research·M K BaumgartnerR S Aronstam

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Citations

Mar 13, 2001·Japanese Journal of Pharmacology·T Fujii, K Kawashima
Mar 28, 2001·Annals of the New York Academy of Sciences·K SchauensteinD A Chambers
Mar 3, 2004·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Takeshi Fujii
Jan 5, 2002·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Y Nomura
Mar 13, 2001·Japanese Journal of Pharmacology·Y Okuma, Y Nomura
Jan 29, 2005·Dementia and Geriatric Cognitive Disorders·Rommy von BernhardiNibaldo C Inestrosa
Sep 11, 2010·The Journal of Biological Chemistry·John F FoleyJoshua M Farber
Sep 30, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·K Peraza-CrucesR Bonfante-Cabarcas
Jul 25, 2012·Annals of the New York Academy of Sciences·Koichiro KawashimaKazuhide Horiguchi
May 4, 2021·Frontiers in Immunology·Namrita Halder, Girdhari Lal
Nov 8, 2001·European Journal of Pharmacology·H Renz
Aug 6, 2002·Journal of Neuroimmunology·Alberto RicciSeyed Khosrow Tayebati
Apr 20, 2010·Biochimica Et Biophysica Acta·Dasiel O Borroto-EscuelaFrancisco Ciruela

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