PMID: 6112051May 18, 1981Paper

Stimulus-dependent labeling of cultured ganglionic cell with [14C]2-deoxyglucose

Brain Research
M Saji, K Obata

Abstract

We prepared cultures of dissociated cells from the ciliary (CG) and dorsal root ganglion (DRG) of 10--12-day-old chick embryos, and applied [14C]2-deoxyglucose ([14C]2-DG) to the cultured cells to examine the effects of stimulation on the labeling with [14C]2-DG at the single cell level. Electrical current stimulation increased [14C]2-DG uptake in CG and DRG neurons. The increase depended on frequency of the stimulation. These effects were potentiated by the application of tetraethylammonium, but suppressed by tetrodotoxin. Externally applied potassium ions increased the [14C]2-DG uptake in the CG cell, depending logarithmically on the concentration of applied KCl. The concentration-dependent increase agreed with potassium effect on the equilibrium potential. For CG cells, acetylcholine (ACh), glutamate, gamma-aminobutyric acid (GABA) and glycine induced remarkable increases of the [14C]2-DG uptake, while dopamine did not induce any change. For DRG cells, GABA and glycine facilitated the [14C]2-DG uptake, while ACh, glutamate and dopamine did not have any significant effects on it. These facilitatory actions of neurotransmitters on the [14C]2-DG uptake are mostly consistent with the excitatory effects of the substrates on both ...Continue Reading

References

Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E J FurshpanD D Potter
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C KennedyL Sokoloff
Jan 1, 1973·The Journal of Physiology·J L Barker, R A Nicoll
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P H O'LagueD D Potter

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Citations

Jan 1, 1983·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A Shainberg, R Pearl
Jan 13, 2000·Journal of Basic and Clinical Physiology and Pharmacology·D el-AniA Shainberg
Jun 8, 1992·The Journal of Comparative Neurology·C L Poitry-Yamate, M Tsacopoulos
Apr 24, 2020·Frontiers in Neuroscience·Bettina U WilkeMichaela Kress

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