Abstract
We prepared cultures of dissociated cells from the ciliary (CG) and dorsal root ganglion (DRG) of 10--12-day-old chick embryos, and applied [14C]2-deoxyglucose ([14C]2-DG) to the cultured cells to examine the effects of stimulation on the labeling with [14C]2-DG at the single cell level. Electrical current stimulation increased [14C]2-DG uptake in CG and DRG neurons. The increase depended on frequency of the stimulation. These effects were potentiated by the application of tetraethylammonium, but suppressed by tetrodotoxin. Externally applied potassium ions increased the [14C]2-DG uptake in the CG cell, depending logarithmically on the concentration of applied KCl. The concentration-dependent increase agreed with potassium effect on the equilibrium potential. For CG cells, acetylcholine (ACh), glutamate, gamma-aminobutyric acid (GABA) and glycine induced remarkable increases of the [14C]2-DG uptake, while dopamine did not induce any change. For DRG cells, GABA and glycine facilitated the [14C]2-DG uptake, while ACh, glutamate and dopamine did not have any significant effects on it. These facilitatory actions of neurotransmitters on the [14C]2-DG uptake are mostly consistent with the excitatory effects of the substrates on both ...Continue Reading
References
Feb 21, 1975·Brain Research·J HooismaW F Stevens
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E J FurshpanD D Potter
Sep 22, 1977·Nature·D H HubelM P Stryker
Apr 28, 1978·Science·M H Des RosiersL Sokoloff
Apr 7, 1978·Brain Research·K ObataH Tanaka
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C KennedyL Sokoloff
Sep 9, 1977·Science·W J Schwartz, H Gainer
Sep 17, 1976·Science·A BrevetF E Stockdale
Mar 7, 1975·Science·C KennedyL Sokoloff
Jun 1, 1972·Developmental Biology·G D Fischbach
Jan 1, 1973·The Journal of Physiology·J L Barker, R A Nicoll
Jun 14, 1974·Brain Research·K Obata
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P H O'LagueD D Potter
Aug 1, 1973·Developmental Biology·J H ChamleyG Burnstock
Feb 1, 1978·The Journal of Cell Biology·M M PooJ W Lam
Citations
May 1, 1990·Neurochemical Research·E DoyleC M Regan
Oct 1, 1986·Journal of Neurocytology·A KuzirianD L Alkon
May 28, 1982·Neuroscience Letters·K Matsunami
May 1, 1989·Brain Research Bulletin·K MatsunamiH Satake
Jan 1, 1983·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A Shainberg, R Pearl
Jan 13, 2000·Journal of Basic and Clinical Physiology and Pharmacology·D el-AniA Shainberg
Jun 6, 1983·Brain Research·J Okada, M Miura
Jun 8, 1992·The Journal of Comparative Neurology·C L Poitry-Yamate, M Tsacopoulos
Apr 24, 2020·Frontiers in Neuroscience·Bettina U WilkeMichaela Kress