Stimulus-dependent modifications in astrocyte-derived extracellular vesicle cargo regulate neuronal excitability

Amrita Datta ChaudhuriNorman J Haughey


Extracellular vesicles have now emerged as key players in cell-to-cell communication. This is particularly important in the central nervous system, where glia-neuron cross-talk helps maintain normal neuronal function. Astrocyte-derived extracellular vesicles (ADEVs) secreted constitutively promote neurite outgrowth and neuronal survival. However, extracellular stimuli can alter the cargo and downstream functions of ADEVs. For example, ADEVs secreted in response to inflammation contain cargo microRNAs and proteins that reduce neurite outgrowth, neuronal firing, and promote neuronal apoptosis. We performed a comprehensive quantitative proteomic analysis to enumerate the proteomic cargo of ADEVs secreted in response to multiple stimuli. Rat primary astrocytes were stimulated with a trophic stimulus (adenosine triphosphate, ATP), an inflammatory stimulus (IL-1β) or an anti-inflammatory stimulus (IL10) and extracellular vesicles secreted within a 2 hr time frame were collected using sequential ultracentrifugation method. ADEVs secreted constitutively without exposure to any stimulus were used a control. A tandem mass tag-based proteomic platform was used to identify and quantify proteins in the ADEVs. Ingenuity pathway analysis was ...Continue Reading


Aug 1, 1992·British Journal of Pharmacology·E M SilinskyS M Vanner
Jul 1, 1992·Journal of Clinical Immunology·M HowardJ de Vries
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W S GriffinC Araoz
Oct 3, 1999·Experimental Neurology·K L BrewerR P Yezierski
Aug 29, 2001·Neuroscience Letters·G M GiladV H Gilad
Apr 20, 2004·Neuroreport·Yue WangKatsutoshi Furukawa
Jul 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Deepak SrinivasanWilma Friedman
Apr 29, 2005·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·L Elliot HongGunvant K Thaker
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fabio BiancoClaudia Verderio
Jun 2, 2005·The European Journal of Neuroscience·Edward T W BamptonJeremy S H Taylor
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Jun 3, 2006·Journal of Cellular Biochemistry·Seyeon Park, Doc-Gyun Jeong
Jul 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lawrence RajendranKai Simons
Mar 22, 2007·Cellular and Molecular Life Sciences : CMLS·G Burnstock
Aug 19, 2007·Developmental Neurobiology·Anna R TaylorCarolanne E Milligan
Mar 28, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Adele J VincentA Jane Roskams
Mar 21, 2009·The EMBO Journal·Fabio BiancoClaudia Verderio
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Arevik MosoianMary E Klotman
May 21, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Evangelia EmmanouilidouKostas Vekrellis
Nov 1, 2007·Proteomics. Clinical Applications·Eva-Maria Krämer-AlbersJacqueline Trotter
Mar 18, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Emma V JonesKeith K Murai
Jul 8, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Man TangRoderick R McInnes
Jul 14, 2011·Cellular and Molecular Neurobiology·Sebok Kumar Halder, Hiroshi Ueda

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