Stimulus-dependent, promoter-specific binding of transcription factor WRKY1 to Its native promoter and the defense-related gene PcPR1-1 in Parsley

The Plant Cell
Franziska TurckImre E Somssich

Abstract

WRKY transcription factors form a large family that plays a role in plant responses to biotic stress and during senescence. Defining in vivo relevant WRKY/promoter relationships has been hampered by the factors' indiscriminate binding to known W box DNA elements and their possible genetic redundance. Employing chromatin immunoprecipitations (ChIP) of cultured cells, we show that parsley (Petroselinum crispum) WRKY1 protein binds to the W boxes of its native promoter as well as to that of PcWRKY3 and the defense-related PR10-class marker gene Pathogenesis-Related1-1 (PcPR1-1). Although present at low concentrations in resting cells, WRKY1 does not appear to play a role in the immediate early gene response upon elicitation because it does not bind to the promoter at this time. Paradoxically, in vivo binding at the PcWRKY1 promoter correlates more with downregulation of gene expression, whereas previous overexpression studies suggested an activating function of WRKY1 on PcWRKY1 expression. By contrast, PcPR1-1 expression remains strong when its promoter is occupied in vivo by WRKY1. Unexpectedly, ChIP revealed that W boxes at promoter sites are constitutively occupied by other WRKY transcription factors, indicating that site recru...Continue Reading

References

May 16, 1994·FEBS Letters·M A CahillA Nordheim
Dec 1, 1996·Nucleic Acids Research·S de PaterJ Kijne
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·E LogemannK Hahlbrock
Apr 8, 2000·Molecular Plant-microbe Interactions : MPMI·C Y KimM J Cho
Apr 29, 2000·Trends in Plant Science·T EulgemI E Somssich
Apr 11, 2001·Trends in Biochemical Sciences·D S Latchman
Nov 28, 2001·The Plant Journal : for Cell and Molecular Biology·C JohnsonJ Arias
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Laurent DeslandesYves Marco
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
May 10, 2002·Genes & Development·Silke Robatzek, Imre E Somssich
Aug 27, 2002·Current Biology : CB·Lianna JohnsonSteven Jacobsen
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Luis Lopez-MolinaNam-Hai Chua
Feb 27, 2003·Plant Molecular Biology·Jixin DongZhixiang Chen
May 15, 2003·Molecular Plant-microbe Interactions : MPMI·Monika KaldeBernadette Lippok
Sep 16, 2003·Current Opinion in Plant Biology·James Z Zhang

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Citations

Aug 10, 2012·Plant Molecular Biology·Huaishun ShenXiping Wang
Sep 17, 2005·Nature Methods·Anne-Valérie GendrelVincent Colot
Nov 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sandy VanderauweraFrank Van Breusegem
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ligang ChenDiqiu Yu
Nov 1, 2005·Molecular Plant-microbe Interactions : MPMI·Frank L H MenkeDaniel F Klessig
Feb 23, 2007·Molecular Plant-microbe Interactions : MPMI·Colleen KnothThomas Eulgem
May 8, 2009·Plant Physiology·Shree P Pandey, Imre E Somssich
Jul 3, 2007·The Plant Cell·Masaki ShimonoHiroshi Takatsuji
Nov 24, 2006·PLoS Pathogens·Thomas Eulgem
Aug 8, 2009·Bioscience, Biotechnology, and Biochemistry·Tetsuya ChujoHisakazu Yamane
Apr 30, 2014·Functional & Integrative Genomics·Leon Van EckNora L V Lapitan

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