Stimulus-secretion coupling of arginine-induced insulin release: comparison with lysine-induced insulin secretion

Endocrinology
A SenerW J Malaisse

Abstract

L-Lysine, like-L-arginine, L-ornithine, or L-homoarginine, accumulated in rat pancreatic islets and stimulated 86Rb efflux, 45Ca uptake and efflux, and insulin release in islets exposed to D-glucose (7.0 mM). The effect of L-lysine differed from that of the other cationic amino acids by such features as the absence of a threshold concentration for stimulation of insulin release, a much lesser sensitivity of the secretory response to intracellular acidification, and the stimulation of 86Rb net uptake over 60 min of incubation. This coincided with the fact that even in the absence of another exogenous nutrient, L-lysine was well oxidized, augmented NH4+ production, increased both the ATP content and ATP/ADP ratio, caused a time-related decrease in 86Rb fractional outflow, and provoked either a transient (10 mM L-lysine) or sustained (20 mM L-lysine) stimulation of insulin secretion. It is proposed, therefore, that the functional response of the pancreatic B-cell to L-lysine involves not only a biophysical mechanism similar to that responsible for the insulinotropic action of L-homoarginine, but also a significant, albeit modest, metabolic component, which reflects the capacity of L-lysine to act as a fuel in islet cells.

Citations

Aug 7, 1992·Biochimica Et Biophysica Acta·M ErecińskaD Nelson
Nov 13, 2004·The Journal of Nutritional Biochemistry·H SoneY Furukawa
Nov 24, 2011·Journal of the International Society of Sports Nutrition·Tsong-Rong JangChen-Kang Chang
Sep 4, 2008·Journal of Cellular Physiology·Mireille AndriamihajaFrançois Blachier
Mar 30, 2006·American Journal of Physiology. Endocrinology and Metabolism·Paul J RozanceWilliam W Hay
Nov 8, 2019·Biochemical Society Transactions·Mauricio Di Fulvio, Lydia Aguilar-Bryan
Sep 2, 1998·The American Journal of Physiology·R Henningsson, I Lundquist
Jun 29, 2000·The American Journal of Clinical Nutrition·L J van LoonA J Wagenmakers
Jan 11, 2007·American Journal of Physiology. Endocrinology and Metabolism·Paul J RozanceWilliam W Hay
Jul 1, 1990·The American Journal of Physiology·W J MalaisseA Sener

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