PMID: 7015071May 1, 1981Paper

Stimulus-secretion coupling of glucose-induced insulin release. Timing of early metabolic, ionic, and secretory events

Metabolism: Clinical and Experimental
W J MalaisseA Sener

Abstract

The timing of the early metabolic, ionic, and secretory responses to glucose in rat pancreatic islets was monitored by measuring, at 12 sec intervals, the concentrations of glucose, lactic, and pyruvic acids, 32P, 86Rb, 45ca, and insulin in the effluent of perifused prelabeled islets. The increase in glucose concentration from zero to 16.7 mM was complete within 133 sec. The output of organic acids increased after 24 sec of exposure to glucose and, in the case of lactic acid, fell slightly after the initial elevation. The phosphate flush was initiated only after 96 sec of exposure to glucose, whereas the decreases in 86Rb and 45Ca outflow were both detectable within 72 sec of stimulation. The secondary rise in 45Ca efflux was first seen after 157 sec of stimulation and its time course was not vastly different from that of insulin release. These data indicate that, in the secretory sequence, metabolic changes precede both the remodelling of ionic fluxes and the stimulation of insulin release. The results are compatible with the view that the secondary rise in 45Ca outflow is attributable, in part at least, to the glucose-induced decrease in K conductance (but not to the increase in phosphate outflow), with resulting membrane dep...Continue Reading

References

Apr 1, 1979·Metabolism: Clinical and Experimental·W J MalaisseJ C Hutton
Jan 1, 1977·Biochemical Society Transactions·W J Malaisse
Mar 20, 1978·Pflügers Archiv : European journal of physiology·W J MalaisseJ C Hutton
Sep 1, 1978·The Journal of Clinical Investigation·B J FrankelG M Grodsky
Apr 28, 1978·Annals of the New York Academy of Sciences·W J MalaisseE Rojas
Oct 1, 1979·Biochemical Medicine·A HerchuelzW J Malaisse
Jan 1, 1976·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·H P Meissner, I J Atwater
Jan 1, 1972·Diabetes·F M MatschinskyJ Kotler-Brajtburg
Oct 1, 1973·Diabetologia·L A Idahl
Nov 1, 1974·The Journal of Clinical Investigation·N FreinkelR M Dawson
Jul 6, 1971·Biochimica Et Biophysica Acta·B HellmanI B Täljedal
Sep 1, 1970·The Journal of Physiology·P M Dean, E K Matthews
Feb 15, 1980·The Biochemical Journal·J C Henquin
Apr 28, 1980·Life Sciences·W J MalaisseA Sener

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Citations

Oct 15, 1984·Experientia·W J MalaisseA Sener
Oct 1, 1985·Biochemical Pharmacology·P C MathiasW J Malaisse
Dec 14, 1989·Biochimica Et Biophysica Acta·E GrapengiesserB Hellman
Dec 1, 1992·Journal of Pharmacological and Toxicological Methods·S el RaziA R Carpinelli
Aug 14, 1998·General Pharmacology·L LadrièreW J Malaisse
Apr 1, 1988·The Journal of Clinical Investigation·B Peter-RieschC B Wollheim
Jan 1, 1986·Diabetes/metabolism Reviews·M D Meglasson, F M Matschinsky
Apr 1, 1986·Cell Biochemistry and Function·A R CarpinelliW J Malaisse
Jun 18, 1998·Annals of the New York Academy of Sciences·N Rideau

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