Stirrer tank: an appropriate technology to immobilize the CB.Hep-1 monoclonal antibody for immunoaffinity purification

Journal of Chromatography. B, Biomedical Sciences and Applications
R HernándezL Herrera

Abstract

The CB.Hep-1 monoclonal antibody was coupled to CNBr-activated Sepharose CL 4B at three different immobilization scales for purification of recombinant hepatitis B surface antigen. Standard laboratory apparatus to obtain immunosorbents of 1 l (scale I) and 3 l (scale II) as well as a stirrer tank to prepare 6 l immunosorbents (scale III) were used. The binding capacity at scale III was 2- and 1.5-fold higher with respect to the scales II and I, while a reduction in the ligand leakage of 5- and 2-folds was observed. Immunosorbents from scale II showed a significantly reduced adsorption, and an increased ligand leakage. Differences in the coupling efficiency were not observed. Antigen purity eluted from the immunosorbents was always above 85%.

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Citations

Jan 31, 2012·Biologicals : Journal of the International Association of Biological Standardization·Lorely MiláWilliams Ferro
May 18, 2013·Biomedical Chromatography : BMC·Lan ZhaoZhiguo Su
Apr 6, 2004·Biochemical and Biophysical Research Communications·Biunayki Reyes DíazMaría Elena Pérez
Jan 25, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Raúl HernándezAlejandro Beldarraín

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