PMID: 6171826Nov 1, 1981Paper

Stochastic expression of fetal hemoglobin in adult erythroid cells

Proceedings of the National Academy of Sciences of the United States of America
G StamatoyannopoulosT Papayannopoulou

Abstract

The expression of fetal hemoglobin, Hb F, in the adult cells is cellularly restricted both in vivo and in culture. Because, in cultures of erythroid progenitors, subclones that express or fail to express Hb F are derived from the same erythroid stem cell, a mechanism must exist whereby Hb F expression segregates in the progeny of erythroid progenitors during their differentiation. We present mathematical analyses of experimental data which suggest that expression of Hb F in human adult erythroid cells occurs on a stochastic basis. We quantified Hb F expression among the subclones of erythroid bursts (clones) in vitro by labeling subclones with fluorescent anti-Hb F antibodies. The observed data were compared with predictions from a stochastic model with the assumption that the expression of Hb F in a subclone occurs with a probability P equal to the frequency of Hb F-expressing subclones in an experiment. There was good fit between the observed and predicted data with respect to: (i) the relative frequencies of monomorphic F+, monomorphic F-, and bimorphic F+/F- bursts, respectively; (ii) the size distributions among F+, F- and F+/F- bursts; and (iii) the proportions of subclones expressing Hb F within bimorphic F+/F- bursts. G...Continue Reading

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S H OrkinP Leder
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·T PapayannopoulouG Stamatoyannopoulos
Jan 12, 1978·The New England Journal of Medicine·T PapayannopoulouG Stamatoyannopoulos
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T H PapayannopoulouG Stamatoyannopoulos
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Jan 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·J E TILLL SIMINOVITCH

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Citations

Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G StamatoyannopoulosT Papayannopoulou
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M GroudineG Stamatoyannopoulos
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E A Raleigh, N Kleckner
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T Kuczek, D E Axelrod
May 1, 1999·Human Gene Therapy·D W EmeryG Stamatoyannopoulos
May 1, 1987·Biological Reviews of the Cambridge Philosophical Society·J Michaelson
Apr 1, 1983·The Journal of Clinical Investigation·R S WeinbergB P Alter
Jan 1, 1985·Annals of the New York Academy of Sciences·D M KurnitS Matthysse
Apr 15, 2011·Blood·Idowu AkinsheyeMartin H Steinberg
Dec 25, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Selda SamakogluJean Michel Heard
Apr 1, 1985·Journal of Cellular Physiology·D M KurnitG Stamatoyannopoulos
Jan 1, 1982·Journal of Cellular Physiology. Supplement·T Papayannopoulou, G Stamatoyannopoulos
Jun 1, 1989·Journal of Cellular Physiology·K Tsuji, T Nakahata
May 12, 2005·Journal of Cellular Physiology·Emery H BresnickKirby D Johnson
Jul 1, 2005·Transfusion·Jeffery L Miller
Jan 1, 1993·American Journal of Hematology·D LavelleP Heller
Dec 12, 2017·Blood Cells, Molecules & Diseases·Tasha A MorrisonDavid H K Chui
Feb 26, 2021·Current Opinion in Hematology·Eugene Khandros, Gerd A Blobel

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