Stochastic nature and red cell population distribution of the sickling-induced Ca2+ permeability

The Journal of Clinical Investigation
V L LewR M Bookchin

Abstract

To explore basic properties of the sickling-induced cation permeability pathway, the Ca2+ component (Psickle-Ca) was studied in density-fractionated sickle cell anemia (SS) discocytes through its effects on the activity of the cells' Ca2+sensitive K+-channels (KCa). The instant state of KCa channel activation was monitored during continuous or cyclic deoxygenation of the cells using a novel thiocyanate-densecell formation method. Each deoxy pulse caused a reversible, sustained Psickle-Ca, which activated KCa channels in only 10-45% of cells at physiological [Ca2+]o ("activated cells"). After removal of cells activated by each previous deoxy pulse, subsequent pulses generated similar activated cell fractions, indicating a random determination rather than the response of a specific vulnerable subpopulation. The fraction of activated cells rose monotonically with [Ca2+]o along a curve reflecting the cells' distribution of Psickle-Ca, with values high enough in a small cell fraction to trigger near-maximal KCa channels. Consistent with the stochastic nature of Psickle-Ca, repeated deoxygenated-oxygenated pulsing led to progressive dense cell formation, whereas single long pulses caused one early density shift. Thus deoxygenation-in...Continue Reading

References

Jan 1, 1991·The Journal of Clinical Investigation·R M BookchinV L Lew
Aug 18, 1988·Biochimica Et Biophysica Acta·T TiffertV L Lew
Nov 9, 1973·Nature·J W EatonH S Jacob
Feb 23, 1996·Journal of Molecular Biology·Z Cao, F A Ferrone
Sep 20, 1955·The Journal of General Physiology·D C TOSTESONE T DUNHAM

❮ Previous
Next ❯

Citations

Jul 16, 2011·Cell Biochemistry and Biophysics·Charles O NwambaAli Akabar Moosavi-Movahedi
Mar 25, 2004·Bioelectrochemistry·Asif I KhanJohn S Gibson
May 2, 2007·The Journal of General Physiology·Teresa TiffertVirgilio L Lew
Feb 15, 2002·Current Opinion in Hematology·Robert M Bookchin, Virgilio L Lew
Aug 18, 2004·Current Opinion in Hematology·Gordon W Stewart
Mar 23, 2013·Current Opinion in Hematology·Patrick G Gallagher
Jun 1, 1997·The Journal of Clinical Investigation·H M Ranney
Jan 1, 2009·Mediterranean Journal of Hematology and Infectious Diseases·Lucia De Franceschi
Jan 9, 2013·The Journal of Physiology·C MilliganJ S Gibson
Mar 25, 2011·Blood Cells, Molecules & Diseases·Serge L Y ThomasCéline Ollivaux
May 6, 2008·Blood Cells, Molecules & Diseases·J C ElloryJ S Gibson
Jun 7, 2008·Bioelectrochemistry·Maria Baunbaek, Poul Bennekou
Apr 17, 2007·Blood Cells, Molecules & Diseases·J C ElloryJ S Gibson
Apr 29, 2006·British Journal of Haematology·Kitty de Jong, Frans A Kuypers
Oct 27, 2006·Pharmacotherapy·Kenneth I AtagaJonathan W Stocker
Jul 17, 2015·American Journal of Hematology·Natasha ArcherCarlo Brugnara
May 23, 2015·ELife·Stuart M CahalanArdem Patapoutian
Sep 7, 2007·Trends in Parasitology·Virgilio L Lew, Teresa Tiffert
May 19, 2001·Blood Cells, Molecules & Diseases·C Brugnara
May 19, 2001·Blood Cells, Molecules & Diseases·J S Gibson
May 24, 2013·International Journal of Molecular Sciences·Anna BogdanovaLars Kaestner
Jun 2, 2017·Physiological Reviews·Nicholas StaffordElizabeth J Cartwright
Jun 26, 2018·British Journal of Haematology·Anke HannemannJohn S Gibson
Oct 21, 2017·Blood·Patrick G Gallagher
Dec 25, 2004·Physiological Reviews·Virgilio L Lew, Robert M Bookchin
Feb 21, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Joseph A BrowningJohn S Gibson
Jan 22, 2019·European Journal of Haematology·Andrea PiccinOwen P Smith
Mar 22, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J S GibsonJ C Ellory

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.