Stoichiometry of complexes between mannose-binding protein and its associated serine proteases. Defining functional units for complement activation

The Journal of Biological Chemistry
C B Chen, Russell Wallis

Abstract

Serum mannose-binding protein (MBP) initiates the lectin branch of the complement cascade by binding to sugars on the surfaces of microorganisms and activating two MBP-associated serine proteases (MASP-1 and MASP-2). Rat serum MBP consists of oligomers containing up to four copies of a subunit that is composed of three identical polypeptide chains. Biophysical analysis of intact and truncated MASPs indicates that each MASP is a homodimer that is stabilized through interactions involving an N-terminal CUB domain. The binding sites for MBP are formed from the three N-terminal MASP domains, in which two CUB modules interact with MBP. Each MASP dimer contains binding sites for two MBP subunits. Both sites must be occupied by subunits from a single MBP oligomer to form a stable complex. Thus, the smallest functional unit for complement activation consists of MBP dimers bound to MASP-1 or MASP-2 homodimers. Trimers and tetramers of MBP form complexes containing up to two MASPs. The results reveal how MASP-1 and MASP-2 can function independently to activate the complement cascade.

References

Sep 1, 1991·Immunology Today·R B Sim, K B Reid
Jan 1, 1990·Methods in Enzymology·R J Kaufman
May 1, 1989·The Journal of Experimental Medicine·M KuhlmanR A Ezekowitz
Oct 29, 1993·Biochemical and Biophysical Research Communications·F TakadaM Kawakami
Jan 1, 1993·Annual Review of Cell Biology·K Drickamer, M E Taylor
Jul 15, 1997·The Biochemical Journal·R Wallis, K Drickamer
Jan 27, 1998·Methods : a Companion to Methods in Enzymology·R K Clyne, T J Kelly
Aug 13, 1998·Immunological Reviews·W I WeisK Drickamer
Jan 28, 1999·The Journal of Biological Chemistry·R Wallis, K Drickamer
Oct 21, 1999·Methods : a Companion to Methods in Enzymology·G RivasA P Minton
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N M ThielensG J Arlaud

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Citations

Apr 9, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Misao MatsushitaTeizo Fujita
Sep 29, 2005·Springer Seminars in Immunopathology·Rikke SørensenJens C Jensenius
Nov 8, 2003·Journal of Immunological Methods·Mette Møller-KristensenSteffen Thiel
Jan 17, 2004·Molecular Immunology·Julia S PresanisRobert B Sim
May 30, 2002·Nature Reviews. Immunology·Teizo Fujita
Aug 9, 2003·The New England Journal of Medicine·Kristian Stengaard-PedersenJens C Jensenius
May 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Richard BerryClair Baldock
Feb 2, 2010·The Journal of Biological Chemistry·Umakhanth Venkatraman GirijaRussell Wallis
Sep 4, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·József DobóPéter Gál
Jan 14, 2003·Annual Review of Immunology·Uffe HolmskovJens C Jensenius
Jun 28, 2011·Advanced Drug Delivery Reviews·Thomas Vorup-Jensen, Thomas Boesen
Nov 27, 2015·Molecular Immunology·Stefanie Epp BoschmannIara J de Messias-Reason
Feb 14, 2008·Biochimica Et Biophysica Acta·Stacy M AlvaresWilliam G Carter
Sep 14, 2007·Biochimie·Renee C DuncanRobert N Pike
May 14, 2004·Scandinavian Journal of Immunology·A SteinøG Houen
Jun 18, 2004·Immunological Reviews·Teizo FujitaYuichi Endo
Dec 25, 2010·European Journal of Immunology·Umakhanth Venkatraman GirijaRussell Wallis
May 16, 2013·Scandinavian Journal of Immunology·S E Degn, S Thiel
Dec 13, 2006·Immunology Letters·Kristelle S BrownTimothy P Hickling
Jun 5, 2007·Immunobiology·Péter GálPéter Závodszky
Jun 5, 2007·Immunobiology·Christine GaboriaudGérard J Arlaud
Jun 5, 2007·Immunobiology·Søren E DegnJens C Jensenius
Aug 30, 2011·Journal of Immunological Methods·Søren E DegnJens C Jensenius
Feb 6, 2015·Frontiers in Pediatrics·Marcia Holsbach BeltrameIara José de Messias-Reason
Apr 23, 2016·Scandinavian Journal of Immunology·T R KjaerS Thiel
Feb 19, 2002·The Journal of Biological Chemistry·Kenneth K-S NgWilliam I Weis
Apr 3, 2004·The Journal of Biological Chemistry·Ce-Belle Chen, Russell Wallis
Sep 16, 2003·Journal of Leukocyte Biology·T P HicklingR B Sim
Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sandor CsehNicole M Thielens
Feb 25, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Florence TeilletNicole M Thielens

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