Stoichiometry of estramustine phosphate binding to MAP2 measured by the disassembly of chick brain MAP2:tubulin microtubules

Cell Motility and the Cytoskeleton
R G Burns

Abstract

The concentration of estramustine phosphate required to inhibit the assembly or to induce the disassembly of chick brain MAP2:tubulin microtubules is markedly dependent upon the microtubule protein concentration. Analysis of this relationship shows that estramustine phosphate and tubulin compete for common MAP2 sites, that MAP2 can bind 5-6 moles.mole-1 estramustine phosphate, and that the Kd of these sites is congruent to 20 microM estramustine phosphate. It is proposed that two molecules of estramustine phosphate interact with each of the three tubulin-binding sites of MAP2 and inhibit the MAP2:tubulin interaction by neutralising two highly conserved basic residues.

References

Jun 1, 1986·The Journal of Cell Biology·S A LewisN J Cowan
Feb 5, 1988·Journal of Molecular Biology·M J Monteiro, D W Cleveland
Jul 1, 1981·European Journal of Biochemistry·R G Burns, K Islam
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L Jameson, M Caplow

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Citations

Feb 1, 1992·Current Opinion in Cell Biology·R F LudueñaI A Khan
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·D PandaL Wilson
Jan 1, 1992·Cell Motility and the Cytoskeleton·B FridénM Wallin
Feb 15, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gary HudesBeryl Hartley-Asp

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