Stoichiometry of reovirus structural proteins in virus, ISVP, and core particles

Virology
K M Coombs

Abstract

All eight reovirus structural proteins were resolved in a new tris, glycine, and urea (TGU) electrophoretic gel system. The specific identities of proteins were determined immunologically, biochemically, and genetically. Structural proteins of reovirus type 1 Lang had different mobilities in the TGU gel than did type 3 Dearing proteins. Intertypic reassortant viruses that contained various combinations of parental genes were used to identify each of the viral protein bands. Type 1 Lang virions were metabolically-labelled with either 3H-amino acids or 35S-methionine/cysteine and gradient purified. Aliquots of purified virions were treated to generate infectious subviral particles (ISVPs) and core particles. Radiolabelled virus, ISVP, and core proteins were resolved in the TGU gel and protein band intensities were used to determine copy numbers of each structural protein. These studies confirmed the copy numbers and locations of most reovirus proteins. However, important new findings include the discovery that virions contain approximately 120 copies of major core protein sigma 2 and 20 copies of the polymerase cofactor protein mu 2, and ISVP particles contain about 24 copies of mu 1 C that has not been processed to the delta pep...Continue Reading

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Citations

Oct 28, 2010·The Journal of Biological Chemistry·Laura S OomsJames D Chappell
Aug 31, 2006·Journal of Virology·Takeshi KobayashiTerence S Dermody
Jan 30, 2009·Journal of Virology·Takeshi KobayashiTerence S Dermody
May 28, 2004·Journal of Virology·Laura L Hermann, Kevin M Coombs
Jul 5, 2002·Journal of Virology·Joseph W GoldenLeslie A Schiff
Dec 13, 2002·Rapid Communications in Mass Spectrometry : RCM·Magdalena I SwansonKevin M Coombs
May 21, 2005·Journal of Virological Methods·Jieyuan Jiang, Kevin M Coombs
Aug 19, 2007·Journal of Pharmaceutical and Biomedical Analysis·Parminder Singh ChahalAmine Kamen
Mar 7, 2008·Journal of Structural Biology·Israel I MendezKevin M Coombs
Jan 1, 2014·Uirusu·Takeshi Kobayashi
Sep 10, 2003·International Reviews of Immunology·Sean M O'DonnellTerence S Dermody
Aug 3, 2018·Journal of Virology·Deepti Thete, Pranav Danthi
Nov 2, 2018·Journal of Virology·Anthony J SnyderPranav Danthi
Feb 23, 2019·Journal of Virology·Anthony J Snyder, Pranav Danthi
Aug 5, 2009·Current Protocols in Microbiology·Alicia Berard, Kevin M Coombs
Oct 22, 2003·Canadian Journal of Microbiology·Etienne Rouault, Guy Lemay

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