Abstract
The extracellular hemoglobin (Hb) of the earthworm, Lumbricus terrestris, has four major O2-binding chains, a, b, c (forming a disulfide-linked trimer), and d ("monomer"). Additional structural chains, "linkers," are required for the assembly of the approximately 200-polypeptide molecule. The proportion of linker chains had been reported to be one-third of the total mass on the basis of densitometry of Coomassie Blue-stained SDS-gels. Reverse-phase high performance liquid chromatography (HPLC), however, gave 16.3% linkers on the basis of both 220-nm absorbance and amino acid analysis (Ownby, D. W., Zhu, H., Schneider, K., Beavis, R. C., Chait, B. C., and Riggs, A. F. (1993) J. Biol. Chem. 268, 13539-13547). The subunit proportions have now been redetermined by SDS capillary electrophoresis as a test of the HPLC results. The electrophoresis, monitored at 214 nm, avoided the use of Coomassie Blue and provided results identical with those obtained by HPLC. Capillary electrophoresis monitored at both 214 and 415 nm was used to show that linker chains do not bind heme. Heme content has been found to be 2.9% by determination of hemin, amino acid analysis and dry weight. Measurement of the rate of hemin loss from oxidized L. terrestri...Continue Reading
References
May 27, 1977·Biochimica Et Biophysica Acta·S N VinogradovH Mizukami
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S N VinogradovD A Walz
May 15, 1986·Analytical Biochemistry·E H Ball
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S N VinogradovA V Crewe
Sep 1, 1986·Archives of Biochemistry and Biophysics·Y Nozaki
May 1, 1965·Analytical Biochemistry·B F Cameron
Jan 1, 1972·Methods in Enzymology·K WeberM Osborn
Apr 29, 1969·Biochemical and Biophysical Research Communications·H Matsubara, R M Sasaki
May 1, 1965·Analytical Biochemistry·A D Adler, P George
Jan 16, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·H U WolfR Zander
Feb 1, 1982·Analytical Biochemistry·S N VinogradovB Zak
Jun 24, 1982·Biochimica Et Biophysica Acta·P Frossard
Jun 24, 1982·Biochimica Et Biophysica Acta·P Frossard
Jan 1, 1995·Journal of Structural Biology·M SchatzM van Heel
Jun 9, 1995·The Journal of Biological Chemistry·R E Benesch, S Kwong
Feb 8, 1996·Biochimica Et Biophysica Acta·K FushitaniK Hosokawa
Nov 22, 1996·The Journal of Biological Chemistry·H ZhuA F Riggs
Jan 1, 1963·Journal of Molecular Biology·O LEVIN
Jun 17, 1960·Biochimica Et Biophysica Acta·J ROCHEJ P THIERY
Jan 1, 1948·Acta Chemica Scandinavica·C DE DUVE
Citations
Mar 23, 2011·Ecotoxicology·A CalisiT Schettino
Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·Serge N Vinogradov
Oct 9, 1999·Biochimica Et Biophysica Acta·Y Tsfadia, E Daniel
Jun 7, 2002·Biophysical Chemistry·Carolina Bosch CabralLewis Joel Greene
Jun 24, 2003·Biochimica Et Biophysica Acta·Ezra DanielYossi Tsfadia
Apr 16, 2011·Biochemistry·Smita KakarMark S Hargrove
Dec 9, 2008·Analytical Biochemistry·Francisco Adriano O CarvalhoMarcel Tabak
Jan 21, 2006·Proteins·Wen-Yen KaoAusten F Riggs
Mar 16, 2012·Journal of Food Science·Jack Appiah Ofori, Yun-Hwa Peggy Hsieh
Jan 18, 2011·Biochimica Et Biophysica Acta·Xinlei QianDerek R Lovley
Sep 10, 1999·Journal of Structural Biology·S LanzavecchiaP L Bellon
Mar 14, 2007·Journal of Structural Biology·Marco GregoriniAndreas Engel
Jun 22, 2005·Expert Review of Proteomics·Gary B Smejkal
Mar 29, 2001·Physiological Reviews·R E Weber, S N Vinogradov
Sep 25, 1999·The Journal of Biological Chemistry·B N GreenS N Vinogradov
Nov 22, 1996·The Journal of Biological Chemistry·H ZhuA F Riggs
Dec 15, 2020·ACS Biomaterials Science & Engineering·Chintan SavlaAndre F Palmer
Apr 7, 2021·Biomacromolecules·Chintan Savla, Andre F Palmer
Jun 10, 1998·Analytical Biochemistry·S L KaufmanS N Vinogradov
Jun 9, 2001·Journal of Molecular Biology·B N GreenS N Vinogradov