PMID: 41590Nov 23, 1979

Stoichiometry of the reaction of oxyhemoglobin with nitrite

Biochimica Et Biophysica Acta
H KosakaI Tyuma


During the reaction of oxyhemoglobin (HbO2) with nitrite, the concentration of residual nitrite, nitrate, oxygen, and methemoglobin (Hb+) was determined successively. The results obtained at various pH values indicate the following stoichiometry for the overall reaction: 4HbO2 + 4NO2- 4H+ leads to 4Hb+ + 4NO3- + O2 + 2H2 O (Hb denotes hemoglobin monomer). NO2- binds with methemoglobin noncooperatively with a binding constant of 340 M-1 at pH 7.4 and 25 degrees C. Thus, the major part of Hb+ produced is aquomethemoglobin, not methemoglobin nitrite, when less than 2 equivalents of nitrite is used for the oxidation.


Feb 1, 1980·International Archives of Occupational and Environmental Health·K ImaizumiY Ueda
Oct 27, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·E Hegesh, J Shiloah
May 1, 1994·Respiration Physiology·J IwamotoF C Morin
Feb 1, 1983·Toxicology and Applied Pharmacology·R L Saul, M C Archer
Jun 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·I R RowlandO C Bøckmann
Jul 14, 1995·Neuroscience Letters·K YamanakaH Kosaka
Aug 1, 1996·Nuclear Medicine and Biology·T J McCarthyM J Welch
Dec 4, 2003·Free Radical Biology & Medicine·Netanya Y SpencerNeil Hogg
May 13, 1999·Biochimica Et Biophysica Acta·Malte Kelm
Jan 10, 2002·European Journal of Pharmacology·M RahmaY Abe
Mar 18, 2000·Neuroscience Research·N Yonehara, M Yoshimura
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L J IgnarroR E Byrns
Jan 22, 2008·The Journal of Biological Chemistry·Agnes KeszlerNeil Hogg
May 8, 2002·The Journal of Clinical Investigation·Tienush RassafMalte Kelm
Feb 14, 2008·Blood·Rainer KiefmannJahar Bhattacharya
Dec 1, 1985·Environmental Health Perspectives·C C Winterbourn
Aug 1, 1987·Environmental Health Perspectives·H Kosaka, I Tyuma
Aug 1, 1987·Environmental Health Perspectives·N MaedaT Shiga
Aug 1, 1987·Environmental Health Perspectives·K Yoshida, K Kasama
Oct 2, 2012·The Review of Scientific Instruments·Weng Kung PengJongyoon Han
Jul 30, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Denisa HathaziRadu Silaghi-Dumitrescu
Aug 11, 2015·Molecular Nutrition & Food Research·Krishnaraj Sinhji RathodAmrita Ahluwalia
Jan 28, 2006·Redox Report : Communications in Free Radical Research·Hans NohlAndrey V Kozlov
Feb 17, 2009·Medicinal Research Reviews·Ernst E van FaassenMark T Gladwin
Apr 3, 2013·Nitric Oxide : Biology and Chemistry·Landon BellaviaDaniel B Kim-Shapiro
Jan 1, 1997·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals· Mansur R Azari Faith M Williams Peter G Blain John W Edwards
May 3, 2011·Free Radical Biology & Medicine·Marcelo F MontenegroJose E Tanus-Santos
Dec 1, 1990·Kidney International·V CattellS Moncada
Dec 9, 2016·PloS One·Toni G PattonKatherine E Horak


Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R BeneschC I Yu

Related Concepts

Oxyhemoglobin Measurement
Methemoglobin Measurement
Nitrite Measurement
Hemoglobin Measurement
Binding (Molecular Function)

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