PMID: 41590Nov 23, 1979

Stoichiometry of the reaction of oxyhemoglobin with nitrite

Biochimica Et Biophysica Acta
H KosakaI Tyuma

Abstract

During the reaction of oxyhemoglobin (HbO2) with nitrite, the concentration of residual nitrite, nitrate, oxygen, and methemoglobin (Hb+) was determined successively. The results obtained at various pH values indicate the following stoichiometry for the overall reaction: 4HbO2 + 4NO2- 4H+ leads to 4Hb+ + 4NO3- + O2 + 2H2 O (Hb denotes hemoglobin monomer). NO2- binds with methemoglobin noncooperatively with a binding constant of 340 M-1 at pH 7.4 and 25 degrees C. Thus, the major part of Hb+ produced is aquomethemoglobin, not methemoglobin nitrite, when less than 2 equivalents of nitrite is used for the oxidation.

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Citations

Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R BeneschC I Yu

Related Concepts

Methemoglobin
Oxyhemoglobin Measurement
Oxidation
Methemoglobin Measurement
Nitrite Measurement
Hemoglobin Measurement
Nitrates
Binding (Molecular Function)
Nitrites
HBG2

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